Abstract
Urinary filarial antigen isolated from urine samples of microfilaraemic patients was analysed for its antigenic activity by immunoblotting and enzyme linked immunosorbent assay techniques. SDS-PAGE fractionation of urinary filarial antigen showed 11 protein bands, of which two showed reactivity with immunoglobulin-G fraction of filarial serum immunoglobulin in immunoblotting. Antigenic analysis of SDS-PAGE fractions of urinary filarial antigen by inhibition enzyme linked immunosorbent assay using filarial serum immunoglobulin-G andWuchereria bancrofti microfilarial excretory-secretory antigen revealed 3 fractions, numbers 5, 6 and 9 with significant activity. In indirect enzyme linked immunosorbent assay using fractions 5 and 6, filarial immunoglobulin-G antibody was detected in about 90% of microfilaraemics, 80% clinical filariasis and 20% of endemic normal individuals. Further, there was no phosphorylcholine epitope in these fractions. Fractions 5 and 6 can be a candidate antigens for the immunodiagnosis of filariasis.
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Abbreviations
- UFA:
-
Urinary filarial antigen
- UFAC2 :
-
UFA column fraction-2
- FSI:
-
filarial serum immunoglobulin
- Wb mf ES:
-
Wuchereria bancrofti microfilarial excretory-secretory
- NCP:
-
nitrocellulose paper
- ELISA:
-
enzyme linked immunosorbent assay
- PC:
-
phosphorylcholine
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Chenthamarakshan, V., Padigel, U.M., Ramaprasad, P. et al. Diagnostic utility of fractionated urinary filarial antigen. J. Biosci. 18, 319–326 (1993). https://doi.org/10.1007/BF02702989
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DOI: https://doi.org/10.1007/BF02702989