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Use of the plasmid pRK 2013 as a vehicle for transposition inAzotobacter vinelandii

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Abstract

Plasmid stability inAzotobacter vinelandii has been determined and a way to introduce transposon into these cells using the plasmid pRK 2013 has been devised. Transposition of both Tn3 and Tn10 has been attained.

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Abbreviations

BNF:

Burk’s nitrogen-free

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Authors and Affiliations

  1. School of Environmental Sciences, Jawaharlal Nehru University, 110 067, New Delhi, India

    S. H. Phadnis & H. K. Das

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  1. S. H. Phadnis
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  2. H. K. Das
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Phadnis, S.H., Das, H.K. Use of the plasmid pRK 2013 as a vehicle for transposition inAzotobacter vinelandii . J. Biosci. 12, 131–135 (1987). https://doi.org/10.1007/BF02702964

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  • DOI: https://doi.org/10.1007/BF02702964

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