Abstract
The free and N-acetyl glucosamine contents, serving as a measure of the amounts of chitosan and chitin respectively, were determined in the chitinase hydrolysates of the cell wall of a wild strain ofNeurospora crassa. Chitinase, obtained from cultures ofSerratia marcescens, could hydrolyse the cell wall completely apart from being capable of hydrolysing preparations of chitin and chitosan. The free and N-acetyl glucosamines, released by chitinase hydrolysis, were determined by a modified Morgan-Elson reaction carried out in the presence and absence of acetic anhydride. The method is capable of estimating chitin and chitosan contents in as little as 100 μg of cell wall material.
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Chen, G. C. and Johnson, B. R. (1983)Appl. Environ. Microbiol.,46, 13.
Davidson, E. A. (1966)Methods Enzymol.,8, 58.
Hackman, R. H. and Goldberg, M. (1981)Anal. Biochem.,110, 277.
Horton, D. and Lineback, D. R. (1965)Methods Carbohydr. Chem.,5, 403.
Jeauniaux, C. (1966)Methods Enzymol.,8, 644.
Kan, M., Suzuki, F. and Kashigawa, H. (1960)Microchem. J.,8, 42.
Lehman, P. T., White, L. O. and Ride, P. J. (1974)Proc. Soc. Gen. Microbiol.,2, 30.
Monaghan, R. L, Eveleigh, D. E., Tewari, R. and Reese, E. (1972)Nature New Biol.,245, 79.
Muzzarelli, R. A. A. (1977)Chitin (Oxford: Pergamon Press).
Roberts, R. L. and Cabib, E. (1982)Anal. Biochem.,127, 402.
Ride, J. P. and Drysdale, R. B. (1972)Physiol. Plant. Pathol.,2, 7.
Schmit, J. C, Edson, C. M. and Brody, S. (1975)J. Bacterial.,122, 1062.
Subramanyam, G., Venkateswerlu, G. and Rao, S. L. N. (1983)Appl. Environ. Microbiol.,46, 585.
Sweeley, C. C., Bentley, R., Makita, M. and Wells, W. W. (1963)J. Am. Chem. Soc.,85, 2497.
Toei, K. and Kohara, T. (1976)Anal. Chim. Acta.,83, 59.
Tsuji, A., Kinoshita, T. and Hoshino, M. (1969)Chem. Pharm. Bull.,17, 1505.
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Subramanyam, C., Rao, S.L.N. An enzymic method for the determination of chitin and chitosan in fungal cell walls. J. Biosci. 12, 125–129 (1987). https://doi.org/10.1007/BF02702963
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DOI: https://doi.org/10.1007/BF02702963