Abstract
The interaction of α-globulin with urea/guanidine hydrochloride was investigated by determining the apparent partial specific volumes of the protein in these solvents. The apparent partial specific volumes were determined both under isomolal and isopotential conditions. The preferential interaction parameter with solvent components calculated were 0.08 and 0.1 g of urea and guanidine hydrochloride respectively per g protein. In both the cases the interaction was not preferential with water. The total binding of denaturant to α-globulin was calculated both for urea and guanidine hydrochloride and the correlation between experimentally determined number of mol of denaturant bound per mol of protein and the total number of peptide bonds and aromatic amino acids were found to be in excellent agreement with each other. The changes in volume upon transferring α-globulin from a salt solution to 8 M urea and 6 M guanidine hydrochloride were also calculated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- GuHCl:
-
Guanidine hydrochloride
- PS buffer:
-
0.1 M phosphate buffer
- pH 6.5:
-
containing 1 M NaCl
References
Aune, K. C. and Tanford, C. (1967)Biochemistry,8, 4579,4586.
Bull, H. B. and Breese, K. (1968)Ann. Biochem. Biophs.,128, 488,497.
Cassassa, E. F. and Eisenberg, H. (1961)J. Phys. Chem.,65, 427.
Cassassa, E. F. and Eisenberg, H. (1966)Adv. Protein.Chem.,19, 287.
Cohen, G. and Eisenberg, H. (1968)Biopolymers,6, 1977.
Edelstein, S. J. and Schachman, H. K. (1967)J. Biol Chem.,242, 306.
Eisenberg, H. (1976) inBiological macromolecules and polyelectrolytes in solutions (Oxford: Clarendon Press).
Green, R. F. and Pace, C. N. (1974)J. Biol. Chem.,249, 5388.
Hvidt, A.., Johansen, G., Linderstrom-Land, K. and Vaslow, F. (1954)C.R.. Trav. Lab. Carlsberg,29, 129.
Inoue, H. and Timasheff, S. N. (1972)Biopolymers,11, 737.
Katz, S. and Miller, J. E. (1971)Biochemistry,10, 3569.
Kielly, W. W. and Harrington, W. F. (1960)Biochim. Biophs. Acta,41, 401.
Krausz, L. M. and Kauzmann, W. (1965)Proc. Natl. Acad. Sci. USA,53, 1234.
Kutz, I. D. (1971)Am. Chem. Soc.,93, 514.
Lakshmi, T. S. and Nandi, P. K. (1978)Int J. Peptide Protein Res.,12, 197.
Lakshmi, T. S. and Nandi, P. K. (1971)Int J. Peptide Protein Res.,10, 120.
Lee, J. C. and Timasheff, S. N. (1974a)Biochemistry,13, 257.
Lee, J. C. and Timasheff, S. N. (1974b)Arch. Biochem. Biophs. 165, 268.
Linderstrom-Lang, K. and Lanz, H. Jr., (1935)C. R. Trav. Lab. Carlsberg,21, 315.
Nath, R., Rao, K. H. and Gin, K. V. (1957)J. Sci. Ind. Res.,16, 228.
Nozaki, Y. and Tanford, C. (1970)Biol. Chem.,245, 1648.
Pace, C. N. (1975)Crit. Revs. Biochem.,3, 1.
Prakash, V. (1976)Studies on the physiochemical properties of sesame (Sesamum indicum) seed protein Ph.D. Thesis, University of Mysore, Mysore.
Prakash, V. and Nandi, P. K. (1976a)Int. J. Peptide Protein Res.,8, 385.
Prakash, V. and Nandi, P. K. (1976b)Indian Biochem. Biophys., (Supplement),13, 12.
Prakash, V. and Nandi, P. K. (1977a)Int. J. Peptide Protein Res.,9, 97.
Prakash, V. and Nandi, P. K. (1977b)Int. J. Peptide Protein Res.,9, 319.
Prakash, V. and Nandi, P. K. (1978)J. Agru Food Chem.,26, 320.
Prakash, V., Nandi, P. K. and Jirgensons, B. (1980)Int. J. Peptide Protein Res.,15, 305.
Prakash, V. (1980) The Proceedings of the Second Congress of FAOB and Golden Jubilee Meeting of the Society of Biological chemists,Abstract (Biopolymers) No. 43, India.
Prakash, V. and Timasheff, S. N. (1981a)Anal. Biochem.,117, 330.
Prakash, V., Loucheux, C., Scheufele, S., Gorbunoff, M. J. and Timasheff, S. N. (1981b)Arch. Biochem. Biophys.,210, 455.
Prakash, V. (1982)J. Biosci., (in press).
Robinson, D. R. and Jencks, W. P. (1965)J. Am. Chem. Soc.,87, 2462.
Reisler, E. and Eisenberg, H. (1969)Biochemistry,8, 4572.
Reithel, F. J. and Sakuar, J. D. (1963)J. Phys. Chem.,67, 2497.
Sallahuddin, A. and Tanford, C. (1970)Biochemistry,9, 1342.
Schachman, H. K. (1957)Methods Enzymol.,4, 32.
Stabinger, H., Leopold, H. and Kratky, O. (1967)Monatsch. Chem.,98, 436.
Scatchard, G. (1946)J. Am. Chem. Soc.,68, 2315.
Sinha, N. K. and Sen, A. (1962)Trans. Bose es. Inst., Calcutta,25, 37.
Stockmayer, W. H. (1950)J. Chem. Phys.,18, 58.
Squire, P. G. and Himmel, M. E. (1979)Arch. Biochem. Biophys.,196, 165.
Tanford, C. (1968)Adv. Protein Chem.,23, 122.
Tanford, C. (1970)Adv. Protein Chem.,24, 2.
Tanford, C. (1961) inPhysical Chemistry of Macromolecules, ed. N. Kerker (New York: John Wiley and Sons) p. 359.
Timasheff, S. N. (1963) inElectromagnetic Scattering, ed. N. Kerker (New York: Pergamon Press) p. 337.
Ulrich, D. V., Kupke, D. W., Beams, J. W. (1964)Proc. Natl Acad. Sci. USA,52, 349.
Ventura, M. M. and Lima, H. T. (1963)An. Acad. Bras. Cienc.,35, 55.
Author information
Authors and Affiliations
Additional information
This work was done at the Biochemistry Department, Brandeis University, Waltham, Massachusetts 02254, USA.
Rights and permissions
About this article
Cite this article
Prakash, V. Partial specific volumes and interactions with solvent components of α-globulin fromSesamum indicum L. in urea and guanidine hydrochloride. J. Biosci. 4, 347–359 (1982). https://doi.org/10.1007/BF02702747
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02702747