Abstract
Analysis of ribosomes and the post ribosomal supernatant fraction of actively growing cells ofThermomyces lanuginosus showed the presence of free 5 S RNA in the supernatant fraction. This 5 S RNA was identical to the ribosomal 5 S RNA in its electrophoretic mobility on 10% Polyacrylamide gel and in its base composition. 5 S RNA from both the sources gave evidence for the presence of diphosphate at the 5’ end. Most of the 5 S RNA that appeared in the cytoplasm was that transported from the nucleus during the isolation. This could be prevented by the use of a hexylene glycol-HEPES buffer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Erdmann, V. A. (1977)Nucleic Acid Res.,4, rl.
Ford, P. J. (1971)Nature,233, 561.
Hayes, F., Vasseur, M., Nikolaev, N., Schlessinger, D., Sriwidada, J., Krol, A. and Branlant, C. (1975)FEBS Lett.,56, 85.
Knight, E. Jr. and Darnell, J. E. (1967)J. Mol. Biol.,28, 491.
Kossman, C. R., Stamato, T. D. and Pettijohn, D. E. (1971)Nature New Biol., 234, 102.
Leibowitz, R. D., Weinberg, R. A. and Penman, S. (1973)J. ’Mol. Biol.,73, 139.
Melton, D. A., DeRobertis, E. M. and Cortese, R. (1980)Nature,284, 143.
Nageswara Rao, J. S. and Cherayil, J. D. (1979)Curr. Sci.,48, 983.
Peacock, A. C. and Dingman, C. W. (1967)Biochemistry,6, 1818.
Rubin, G. M. and Hogness, D. S. (1975)Cell,6, 207.
Takai, K., Hashimoto, S. and Muramatsu, M. (1975)Biochemistry,14, 536.
Wray, W., Conn, P. M. and Wray, V. P. (1977)Methods Cell Biol.,16, 69.
Wreschner, D. H. (1978)FEBS Lett.,94, 145.
Zahavi-Willner, T. and Danon, D. (1972)FEBS Lett.,26, 151.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nageswara Rao, S., Cherayil, J.D. Evidence for non-coordinated synthesis of 5 S RNA in the thermophilic fungus,Thermomyces lanuginosus . J Biosci 4, 239–244 (1982). https://doi.org/10.1007/BF02702734
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02702734