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Galactose-1-phosphate uridyltransferase of lemurs: A comparison of four species

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Abstract

Erythrocyte galactose-1-phosphate uridyltransferase (Gal-1-PUT) was studied in four species of lemurs. Electrophoretic phenotypes of Lemur fulvusand Lemur macacowere indistinguishable and different from the phenotypes of Lemur cattaand Lemur variegatus,which were different from each other. Enzymatic activity of hemolysates was species specific, with that of Lemur variegatusabout twice that of either Lemur fulvusor Lemur macacoand almost three times that of Lemur catta.Only minor interspecific differences were demonstrated in pH optima and km for galactose-1-phosphate; however, thermal stability varied considerably with phenotype. Antibody inhibition studies indicated that differences in enzyme activity of hemolysates from these species are probably due to differences in enzyme concentration.

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References

  • Barnicot, N. A., and Cohen, P. (1970). Red cell enzymes of primates (Anthropoidea).Biochem. Genet. 4:41–57.

    Article  PubMed  CAS  Google Scholar 

  • Barnicot, N. A., and Hewett-Emmett, D. (1971). Red cell and serum proteins of talapoin, patas and vervet monkeys.Folia Prima tol. 15:65–76.

    Article  CAS  Google Scholar 

  • Beutler, E. (1969). Galactose-1-phosphate uridyl transferase. In Junis, J.J. (ed.).Biochemical Methods in Red Cell Genetics, Academic Press, New York, pp. 289–305.

    Google Scholar 

  • Giblett, E. R. (1969).Genetic Markers in Human Blood, Blackwell, Oxford and Edinburgh.

    Google Scholar 

  • Kendall, F. E. (1937). Studies on serum proteins. I. Identification of a single serum globulin by immunological means. Its distribution in the sera of normal individuals and of patients with cirrhosis of the liver and with glomerulonephritis.J. Clin. Invest. 16: 921–931.

    Article  PubMed  CAS  Google Scholar 

  • Kull, F. C. (1978).Immunological Studies on Variant Human Red Cell Galactose-1-phosphate Uridyltransferase, Ph.D. dissertation, University of North Carolina, Chapel Hill.

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193: 265–275.

    PubMed  CAS  Google Scholar 

  • Mason, G. A., and Buettner-Janusch, J. (1977). Codominant autosomal inheritance of polymorphic red cell acid phosphatases of lemurs and some properties of the enzymes.Biochem. Genet. 15:487–507.

    Article  PubMed  CAS  Google Scholar 

  • Ng, W. G., Bergren, W. R., Fields, M., and Donnell, G. N. (1969). An improved electrophoretic procedure for galactose-1-phosphate uridyltransferase: Demonstration of multiple activity bands with the Duarte variant.Biochem. Biophys. Res. Commun. 37: 354–362.

    Article  CAS  Google Scholar 

  • Prychodko, W., Goodman, M., Singal, B. M., Weiss, M. L., Ishimoto, G., and Tanaka, T. (1971). Starch-gel electrophoretic variants of erythrocyte 6-phosphogluconate dehydrogenase in Asian macaques.Primates 12: 175–182.

    Article  CAS  Google Scholar 

  • Schmitt, J., Lichte, K. H., and Fuhrmann, W. (1970). Red cell enzymes of the Pongidae.Hum. Genet. 10: 138–144.

    Article  CAS  Google Scholar 

  • Yunis, J. J. (1969).Biochemical Methods in Red Cell Genetics, Academic Press, New York and London.

    Google Scholar 

  • Wintrobe, M. M. (1967).Clinical Hematology, Lea and Febinger, Philadelphia.

    Google Scholar 

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Mason, G.A., Frazier, D.M. & Kull, F.C. Galactose-1-phosphate uridyltransferase of lemurs: A comparison of four species. Int J Primatol 1, 15–19 (1980). https://doi.org/10.1007/BF02692255

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