Summary
Cu(II) Co(II) and Fe(II) in pharmaceutical preparations were determined using precolumn derivatization and solvent extraction with 2-acetylpyridine-4-phenyl-3-thiosemicarbazone (APPT) as complexing reagent. Liquid chromatography (LC) was carried out on a reversed phase C-18, column. The complexes were eluted isocratically with acetonitrile-water containing sodium acetate and tetrabutyl ammonium bromide as ion-pairing agents. The results obtained were compared with those using atomic absorption.
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Khuhawar, M.Y., Memon, Z.P. & Lanjwani, S.N. HPLC determination of copper(II), cobalt(II) and iron(II) in pharmaceutical preparations using 2-acetylpyridine-4-phenyl-3-thiosemicarbazone derivatizing agent. Chromatographia 41, 236–237 (1995). https://doi.org/10.1007/BF02688031
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DOI: https://doi.org/10.1007/BF02688031