Skip to main content
SpringerLink
Log in

A normal phase HPTLC method for the quantitative determination of fumonisin B1 in rice

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A normal phase HPTLC method has been validated by spiking, in quadruplet, uncontaminated extract of rice with fumonisin B1 over the range 0 to 16 µg/g. The method utilises solid phase extraction using strong anionic exchange (SAX) cartridges, uni-directional normal phase high performance thin layer chromatography (HPTLC), novel visualisation by dipping into a 0.16 % acidic solution ofp-anisaldehyde and quantification by scanning fluoro-densitometry. Response was linear only over the range 0 to 5 µg/g (0 to 125 ng/spot) where recoveries averaged 81 % for rice. Weighted linear regression yielded a limit of detection of 0.25 µg/g for rice. Coefficients of variance were 15.4, 5.3, 2.8, 3.5, and 0.9 % at fumonisin B1 levels of 0.20, 0.5, 1.0, 2.0 and 5 µg/g respectively, demonstrating good precision. This method claims to be the first fully quantitative HPTLC method for determining fumonisin B1 in rice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. W. C. A. Gelderblom, K. Jaskiewicz, W. F. O Marasas, P. G. Thiel, R. M. Horak, R. Vleggaar, N. P. J. Kriek, Appl. Environ. Microbiol.54, 1806 (1988).

    CAS  Google Scholar 

  2. E. W. Sydenham, W. C. A. Gelderblom, P. G. Thiel, W. F. O Marasas, J. Agr. Food Chem.38, 285 (1990).

    Article  CAS  Google Scholar 

  3. G. E. Rottinghaus, C E Coatney, H C Minor, J Vet. Diagn. Invest.4, 326 (1992).

    Article  CAS  Google Scholar 

  4. A. Pittet, V. Parisod, M. Schellenberg, J. Agr. Food Chem.40, 1352 (1992).

    Article  CAS  Google Scholar 

  5. E. W. Sydenham, W. F. O. Marasas, G. S. Shepard, P. G. Thiel, E. Y. Hirooka, J. Agr. Food Chem.40, 994 (1992).

    Article  CAS  Google Scholar 

  6. W. C. A. Gelderblom, N. P. J. Kriek, W. F. O. Marasas, P. G. Thiel, Carcinogenesis12, 1247 (1991).

    Article  CAS  Google Scholar 

  7. W. F. O. Marasas, T. S. Kellerman, W. C. A. Gelderblom, Onderstepoort J. Vet. Res.55, 197 (1988).

    CAS  Google Scholar 

  8. L. R. Harrison, B. M. Colvin, J. T. Greene, L. E. Newman, R. J. Cole Jr, J. Vet. Diagn. Invest.2, 217 (1990).

    Article  CAS  Google Scholar 

  9. E. W. Sydenham, G. S. Shephard, P G Thiel, Journal Of AOAC International75, 313 (1992).

    CAS  Google Scholar 

  10. R. D. Plattner, W. P. Norred, C. W. Bacon, K. A. Voss, R. Patterson, D. D. Shackelford, D. Weisleder, Mycologia82, 698 (1990).

    Article  CAS  Google Scholar 

  11. K. A. Voss, W. P. Norred, R. D. Plattner, C. W. Bacon, Food Chem. Toxicol.27, 89 (1989).

    Article  CAS  Google Scholar 

  12. J. Le Bars, P. Le Bars, J. Dupuy, H. Boudra, R. Cassini, Proceedings 106th Annu. Assoc. Off. Anal. Chem. Meet., Cincinnati, Ohio, 1992, p. 106.

  13. J. C. Miller, J. N. Miller, “Statistics for Analytical Chemistry”, Ellis Horwood, Chichester, 1988.

    Google Scholar 

  14. M. E. Cawood, W. C. A. Gelderblom, R. Vleggaar, Y. Behrend, P. G. Thiel, W. F. O. Marasas, J. Agr. Food Chem.39, 1958 (1991).

    Article  CAS  Google Scholar 

  15. S. Stockenstrom, E. W. Sydenham, P. G. Thiel, Mycotoxin Research10, 9 (1994).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, New Elephant Road, 1205, Dhaka, Bangladesh

    M. Dawlatana

  2. Natural Resources Institute, Chatham Maritime, Central Avenue, ME4 4TB, Chatham, Kent, UK

    R. D. Coker & M. J. Nagler

  3. School of Pharmacy and Biomedical Sciences, University of Portsmouth, King Henry I Street, PO1 2DZ, Portsmouth, UK

    G. Blunden

Authors
  1. M. Dawlatana
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. D. Coker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. J. Nagler
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Blunden
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dawlatana, M., Coker, R.D., Nagler, M.J. et al. A normal phase HPTLC method for the quantitative determination of fumonisin B1 in rice. Chromatographia 41, 187–190 (1995). https://doi.org/10.1007/BF02688023

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02688023

Key Words

Search

Navigation