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Improved detection of steroids on NH2 layers

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Summary

Thin-layer chromatography (TLC) using NH2-modified TLC plates is a reproducible method for the measurement of steroids. The sensitivity of determination of steroids (cortisol, cortisone, testosterone, progesterone) by measuring fluorescence in situ can be increased twofold if chromatograms are dipped into a mixture of hexane-paraffin. Fluorescence is further increased if filters are used, which cut emission wavelengths below 400 nm. Using the modifications described, steroid concentrations of about 5 ng per spot could be accurately determined. The increased sensitivity allows measurement of the plasma levels of cortisol in small laboratory animals such as guinea pigs.

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Authors and Affiliations

  1. Department of Animal Physiology, University of Bayreuth, 95440, Bayreuth, Germany

    M. Fenske

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  1. M. Fenske
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Fenske, M. Improved detection of steroids on NH2 layers. Chromatographia 41, 175–177 (1995). https://doi.org/10.1007/BF02688020

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  • DOI: https://doi.org/10.1007/BF02688020

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