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Convenient estimation of the mobile phase volume for water-rich eluents in reversed-phase liquid chromatography

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Summary

Oxalodihydroxamic acid is proposed as a UV-detectable substitute for D2O for the convenient estimation of the mobile phase volume (Vm) of water-rich hydro-organic eluents in reversed-phase liquid chromatography. The retention volume of oxalodihydroxamic acid deviates less than 6% from that of D2O in 0 to 50% methanol-and acetonitrile-water eluents on C1, C8, and C18 reversed-phase supports; it is independent of the amount injected from 10ng to 20µg when monitored at 254nm, although the sensitivity is about four-fold greater at 219 nm. The pH should be maintained between about 2 and 4. The deviation from the D2O retention volume on supports of typical porosity is attributed, at least in part, to a size-exclusion effect. Simple hydroxamic acids can be used as a homologous series for the estimation of Vm over most of the methanol- and acetonitrile-water concentration ranges by the linearization of retentions of homologous series method.

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Authors and Affiliations

  1. Department of Chemistry, California State Polytechnic University, 91768, Pomona, CA, USA

    G. Gutnikov & Lang-Bang Hung

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  1. G. Gutnikov
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  2. Lang-Bang Hung
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Gutnikov, G., Hung, LB. Convenient estimation of the mobile phase volume for water-rich eluents in reversed-phase liquid chromatography. Chromatographia 19, 260–265 (1984). https://doi.org/10.1007/BF02687750

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  • DOI: https://doi.org/10.1007/BF02687750

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