Skip to main content
SpringerLink
Log in

Study on the efficiency of assembled packed microbore columns in HPLC

  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Stainless steel columns (internally mirror-finished, 125 or 250nm in length, of bore 1.0 or 1.6mm) were slurry-packed with 5µm and 4µm reversed phase silicas (Hypersil ODS, LiChrosorb RP-8 and RP-18 and Superspher RP-8. The HPLC equipment consisted of a pump LC5A (Shimadzu) or a pump 2150 (LKB Instruments), a rheodyne valve 7413 with loops of 0.5, 1.0 and 5.0mm3 and a Jasco-Uvidec 100-II UV detector, variously with one of three specially constructed cells of 0.2, 0.4 and 1.3mm3 volume. Columns were assembled using two types of coupling device employing a stainless steel capillary of 0.12mm bore. The effect of sample volume, design of coupling device in assembled column, detector cell volume and geometry on column plate count was examined to optimize the conditions and the instrument compartments. The highest efficiency for the assembled columns was achieved by use of the 0.2mm3 detector cell, a low dead volume coupling device and a 0.5µl sample volume. Sets of 5 assembled columns gave reduced plate height values between 2–3 at a reduced velocity 2<v<3, indicating that extra-column effects were negligibly small; the performance was identical to that of conventional analytical columns.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. A. Borman, Anal. Chem.,56, 1031 A (1984).

  2. J. H. Knox, J. Chrom. Sci.,18, 453 (1980).

    CAS  Google Scholar 

  3. R. P. W. Scott, inJ.C. Giddings, E. Grushka, J. Cazes, Ph. R. Brown, Advances in Chromatography, Vol. 22 (1984). M. Dekker, New York, pp. 247–294.

    Google Scholar 

  4. P. Kucera, J. Chromatogr. Library, Vol. 28 (1984), Elsevier, Amsterdam.

    Google Scholar 

  5. R. P. W. Scott, P. Kucera, M. Munroe, J. Chrom.,186, 475 (1979).

    Article  CAS  Google Scholar 

  6. J. J. Kirkland, W. W. Yau, H. J. Stocklosa, C. H. Dilks, J. Chrom. Sci.,15, 303 (1977).

    CAS  Google Scholar 

  7. J. H. Knox, High Performance Liquid Chromatography, Edinburgh University Press (1978), Edinburgh, p. 80.

    Google Scholar 

  8. R. P. W. Scott, P. Kucera, J. Chrom.,185, 27 (1979).

    Article  CAS  Google Scholar 

  9. Sir G. Taylor, Proc. Roy. Soc.,A219, 186 (1953).

    CAS  Google Scholar 

  10. G. Guiochon, H. Colin, inP. Kucera (editor), Microcolumn high-performance liquid chromatography, J. Chrom. Library, Vol. 28 (1984), 1–38, Elsevier, Amsterdam.

    Google Scholar 

  11. Data taken from diploma thesis ofM. Chatziathanassiou, Johannes Gutenberg-Universität, Mainz (1982).

Download references

Author information

Authors and Affiliations

  1. Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität, 6500, Mainz, FRG

    P. Roumeliotis, M. Chatziathanassiou & K. K. Unger

Authors
  1. P. Roumeliotis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Chatziathanassiou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. K. Unger
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roumeliotis, P., Chatziathanassiou, M. & Unger, K.K. Study on the efficiency of assembled packed microbore columns in HPLC. Chromatographia 19, 145–150 (1984). https://doi.org/10.1007/BF02687730

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02687730

Key Words

Search

Navigation