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Preparative isolation of oligodeoxynucleotides

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Summary

DNA can be degradated on a preparative scale to mixtures of oligonucleotides by various chemical methods. The resulting highly complex oligonucleotide mixture can be almost completely separated by liquid chromatography. The separation procedures are demonstrated by the isolation of pyrimidine oligonucleotides from a partial hydrolysate of herring sperm DNA. The partial hydrolysate is fractionated into oligonucleotide mixtures of defined compositions by a separation route in which ion exchangers are used at different pH-values. From the resulting mixtures of sequence isomers pyrimidine oligonucleotides of defined sequences are obtained by ion exchange and/or reversed phase HPLC. Oligonucleotides which can not be isolated by this separation route are obtained from the preseparated partial hydrolysate by template chromatography using the specificity of the base-pairing mechanism. Purity and sequence of the isolated oligonucleotides are determined by the “fingerprint” method.

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  1. Institut für Organische Chemie, Universität Tübingen, Auf der Morgenstelle 18, D-7400, Tübingen, FRG

    H. Schott

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Schott, H. Preparative isolation of oligodeoxynucleotides. Chromatographia 19, 67–76 (1984). https://doi.org/10.1007/BF02687721

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  • DOI: https://doi.org/10.1007/BF02687721

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