Abstract
To improve the efficiency of the use of nuclease P1, enzyme immobilization technology was applied using nuclease P1. Characterization of immobilized nuclease P1 on different supports was studied. The results showed that the optimum pH and temperature of nuclease P1 immobilized via different supports were enhanced. The immobilized enzyme was obviously stable when stored for long periods and was reusable. The best results were obtained when nuclease P1 was immobilized on chitosan nanoparticles. The nanoparticles were applied to protect the activity of nuclease P1 and improved enzyme activity by 13.17% over that of free nuclease P1 at the same conditions. The Michaelis constant Km and V max were determined for free and immobilized enzyme as well.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Juang, R. S., Wu, F. C., and Tseng, R. L. (2001), Bioresour. Technol. 80, 187–193.
Denkbas, E. B., Kilicay, E., Birlikseven, C., and Ozturk, E. (2002), React. Funct. Polym. 50, 225–232.
Selmer-Olsen, E., Ratnaweera, H. C., and Pehrson, R. (1996), Water Sci. Technol. 34, 33–40.
Kucera, J. (2004), J. Chromatogr. B 808, 69–73.
Chiou, S. H. and Wu, W. T. (2004), Biomaterials 25, 197–204.
Mi, F. L., Kuan, C. Y., Shyu, S. S., Lee, S. T., and Chang, S. F. (2000), Carbohydr. Polym. 41, 389–396.
Kuninaka, A., Kibi, M., Yoshino, H., and Sagaguchi, K. (1961), Agric. Biol. Chem. 25, 693–701.
Steensma, A., van Dijck, P. W. M. and Hempenius, R. A. (2004), Food Chem. Toxicol. 42, 935–944.
Kuninaka, A. (1996), in Biotechnology, 2nd ed., vol. 6, Rehm, H. J. and Reed, G., eds., VCH, Germany, pp. 561–612.
Gerald, R. and Tilak, W. (1991), in Yeast Technology, 2nd ed., AVI, New York, pp. 369–412.
Ying, G. Q., Shi, L.-E., Tang, Z. X., and Shan, J. F. (2004), Food Res. Dev. 25, 120–123.
Duran, N., Rosa, M. A., D'Annibale, A., and Gianfreda, L. (2002), Enzyme Microb. Technol. 31, 907–931.
Juang, R. S., Wu, F. C., and Tseng, R. L. (2002), Adv. Environ. Res. 6, 171–177.
Berthold, A., Cremer, K., and Kreuter, J. (1996), J. Control. Release 39, 17–25.
Tian, X. X. and Groves, M. J. (1999), J. Pharm. Pharmacol. 51, 151–157.
Cui, Z. and Mumper, R. J. (2001), J. Control. Release 75, 409–419.
Fujishima, T., Uchida, K., and Yushino, H. (1972), J. Ferment. Technol. 50, 724–730.
Fujimoto, M., Kuninaka, A., and Yoshino, H. (1974), Agric. Biol. Chem. 38, 777–783.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ying, QQ., Shi, LE., Zhang, XY. et al. Characterization of immobilized nuclease P1. Appl Biochem Biotechnol 136, 119–126 (2007). https://doi.org/10.1007/BF02685942
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02685942