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Fluorophore-assisted carbohydrate electrophoresis as detection method for carbohydrate-protein interactions

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Abstract

Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straight-forward, sensitive method for determining the presence and relative abundance of individual (oligo) saccharide in a(n) (oligo) saccharide mixture. The single terminal aldehydes of (oligo) saccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate (ANTS), and separated with high resolution on the basis of size by polyacrylamide gel electrophoresis. ANTS fluorescence labeling is not biased by (oligo) saccharide length. Therefore, band fluorescence intensity is directly related to the relative abundance of individual (oligo) saccharide moieties in heterogeneous sample. In the same time, it also indicates that FACE can be used to investigate the interactions of carbohydrates and proteins.

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology (SKLMT), Shandong University, Jinan, 250100, Shandong, China

    Gang-Liang Huang & Xin-Ya Mei

  2. Department of Immunology, Tongji Medical College, Huazhong, University of Science and Technology, Wuhan, P.R. China

    Heng Yang & Yu-Ting Ma

  3. College of Life Science and Technology, Huazhong University of Science and Technology (East Campus), 1037 Luoyu Road, 430074, Wuhan, China

    Man-Xi Liu

Authors
  1. Gang-Liang Huang
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  2. Heng Yang
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  3. Xin-Ya Mei
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  4. Man-Xi Liu
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  5. Yu-Ting Ma
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Correspondence to Gang-Liang Huang.

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Huang, GL., Yang, H., Mei, XY. et al. Fluorophore-assisted carbohydrate electrophoresis as detection method for carbohydrate-protein interactions. Appl Biochem Biotechnol 136, 17–22 (2007). https://doi.org/10.1007/BF02685935

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  • DOI: https://doi.org/10.1007/BF02685935

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