Abstract
A crude nuclear fraction of resting wheat embryos was used as the source of putative plant minichromosomes: unique DNA sequences the size of genes and flanked by telomere-type repeats. Preliminary separation of low-molecular-weight DNA species from chromosomal DNA (Hirt's method), velocity sedimentation, and isopycnic centrifugation were followed by PCR amplification of minichromosome-like sequences. The most abundant PCR product was cloned and sequenced. In addition to telomeric repeats (defined by a PCR primer), which were the expected sequences, the linear DNA molecule (637 pb) contained an ARS-like element, RAP1-binding site, and two relatively long ORFs. The whole sequence seems to represent a naturally occurring plant minichromosome.
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Abbreviations
- ARS:
-
autonomously replicating sequence
- ORF:
-
open reading frame
- RAP1:
-
repressor/activator protein 1
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Bucholc, M., Buchowicz, J. A minichromosome-like structure in wheat embryos. Plant Mol Biol Rep 12, 204–208 (1994). https://doi.org/10.1007/BF02668742
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DOI: https://doi.org/10.1007/BF02668742