Skip to main content
Log in

Conjugated dienes of crude soy oil: Detection by UV spectrophotometry and separation by HPLC

  • Technical
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

A study of crude soy oils by ultraviolet spectrophotometry showed E 1%1cm values at 233 nm ranging from 1.6 to 2.0. These E 1%1cm values can be used as a measure of the conjugated diene compounds of soy oil. E 1%1cm values for purified triglycerides were 0.78 (tristearin), 1.1 (triolein), and 0.71 (trilinolein). For the triglyceride fraction of crude soy oil after separation by high performance liquid chromatography (HPLC) the E 1%1cm was 0.98. HPLC separation of components in crude soy oil led to a tentative identification of a triglyceride fraction, a carotene fraction, 2 tocopherol fractions, and 4 conjugated diene fractions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Ong, V.T.L., Fette Seifen Anstrichm. 82:169 (1980).

    Article  CAS  Google Scholar 

  2. Brice, B.A., M.L. Swain, S.F. Herb, P.L. Nichols, Jr., and R.W. Riemanschneider, JAOCS 29:279 (1952).

    Article  CAS  Google Scholar 

  3. Golumbic, C., C.J. Martin and B.F. Dubert, Oil Soap (Chicago) 23:187 (1946).

    Article  CAS  Google Scholar 

  4. Golumbic, C., C.J. Martin and B.F. Dubert, Ibid. 23:360 (1946).

    Article  CAS  Google Scholar 

  5. O'Connor, R.T., E.T. Field, M.E. Jefferson and F.G. Dollear, Ibid. 58:575 (1981).

    Google Scholar 

  6. Van Den Bosch, E., Ibid. 50:421 (1973).

    Google Scholar 

  7. St. Angelo, A., R.L. Ory and L.E. Brown, Ibid. 52:34 (1975).

    CAS  Google Scholar 

  8. DuPlessis, L.M., P. Van Twisk, P.J. Van Niekerk and M. Steyn, JAOCS 58:575 (1981).

    Article  CAS  Google Scholar 

  9. Fishwick, M.J., and P.A.T. Swoboda, J. Sci. Food Agric. 28: 387 (1977).

    Article  CAS  Google Scholar 

  10. AOCS Official and Tentative Methods, 3rd edn., American Oil Chemists' Soc., Champaign, IL, 1980 Revision, Method Cd 7~58.

  11. Hirsch, J., and E.H. Ahrens, Jr., J. Biol. Chem. 233:311 (1958).

    CAS  Google Scholar 

  12. Carpenter, A.P., Jr., JAOCS 56:668 (1979).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Published with the approval of the Director of the Arkansas Agricultural Experiment Station.

About this article

Cite this article

Brown, H.G., Snyder, H.E. Conjugated dienes of crude soy oil: Detection by UV spectrophotometry and separation by HPLC. J Am Oil Chem Soc 59, 280–283 (1982). https://doi.org/10.1007/BF02662225

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02662225

Keywords

Navigation