Abstract
A silver-ion high-performance liquid chromatography column (hexane/acetonitrile as solvent, ultraviolet detection) was used to analyze the fatty acid distribution (as fatty acid methyl esters) of a representative sample of hydrogenated oil. Fractions containingcis- andtrans-18:1 isomers were readily separated. The positional fatty acid isomers were separated by rechromatographing these fractions. The elution order and percent compositions were compared with results obtained by gas chromatography. Of the Δ8 to Δ14trans-18:1 isomers, only the Δ8 and Δ9 pair could not be separated. The Δ8 and Δ9cis-18:1 pair also could not be separated, and the Δ10 isomer was poorly separated from this pair. Area percents were comparable to results obtained by gas chromatography.
Similar content being viewed by others
References
Slover, H.T., R.H. Thompson, C.S. Davis and G.V. Merola,J. Am. Oil Chem. Soc. 62:775 (1985).
Mossoba, M.M., R.E. McDonald, D.J. Armstrong and S.W. Page,J. Agric. Food Chem. 39:695 (1991).
Ulberth, F., and M. Henninger,J. Am. Oil Chem. Soc. 69:829 (1992).
Evershed, R.P., inLipid Analysis, A Practical Approach, edited by R.J. Hamilton, and S. Hamilton, Oxford University Press, New York, 1993, pp. 130–133.
Christie, W.W.,Lipid Analysis, 2nd edn., Pergamon Press, 1982, p. 53.
Ratnayake, W.M.N., R. Hollywood, E. O’Grady and J.L. Beare-Rogers,J. Am. Oil Chem. Soc. 67:804 (1990).
Official Methods and Recommended Practices of the American Oil Chemists’ Society, 4th edn., edited by D. Firestone, American Oil Chemists’ Society, Champaign, 1990, Method Ce 1c-89.
Ratnayake, W.M.N., and G. Pelletier,J. Am. Oil Chem. Soc. 69:95 (1992).
Wolff, R.L.,J. Chromatogr. Sci. 30:17 (1992).
Wolff, R.L.,J. Am. Oil Chem. Soc. 69:106 (1992).
Ratnayake, W.M.N., Ibid.:192 (1992).
Roehm, J.N., and O.S. Privett,J. Lipid Res. 10:245 (1969).
Christie, W.W.,Advances in Lipid Methodology—One, The Oily Press, Scotland, 1992, p. 89.
Adlof, R.O.,J. Chromatogr. A 659:95 (1994).
Christie, W.W., and G.H. McG. Breckenridge, Ibid.:261 (1989).
Christie, W.W.,J. High Resolut. Chromatogr. Commun. 10:148 (1987).
Christie, W.W., E.Y. Brechany and K. Stefanov,Chem. Phys. Lipids 46:127 (1988).
Nikolova-Damyanova, B., B.G. Herslof and W.W. Christie, Ibid.:133 (1992).
Toschi, T.G., P. Capella, C. Holt and W.W. Christie,J. Sci. Food Agric. 61:261 (1993).
Butterfield, R.O., W.K. Rohwedder, E.D. Bitner, J.O. Ernst, D.J. Wolf and H.J. Dutton,Prog. Lip. Res. 17:93 (1978).
Beyers, E.C., and E.A. Emken,Biochim. Biophys. Acta 1082:275 (1991).
Author information
Authors and Affiliations
About this article
Cite this article
Adlof, R.O., Copes, L.C. & Emken, E.A. Analysis of the monoenoic fatty acid distribution in hydrogenated vegetable oils by silver-ion high-performance liquid chromatography. J Am Oil Chem Soc 72, 571–574 (1995). https://doi.org/10.1007/BF02638858
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02638858