Summary
The lipase content of rapeseed germinated in light increases by 100-fold over that of the dormant seed. The increase was much less in seeds germinated in the absence of light. A zero-order reaction rate for the enzyme was observed when tributyrin was employed as the substrate. Optimum activity occurred at pH 8.5. The Michaelis-Menton was calculated to be 4.06×10−4 M, which is close to values reported for other lipases. While most substances tested had no effect on fat-splitting activity, NaCN, NaN3, and glutathione behaved as activators.
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Contribution from the National Research Council of Canada, Prairie Regional Laboratory, Saskatoon, Saskatchewan. Issued as Paper No. 236 on the “Uses of Plant Products” and as N.R.C. No. 4175.
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Wetter, L.R. Some properties of the lipase present in germinating rapeseed. J Am Oil Chem Soc 34, 66–70 (1957). https://doi.org/10.1007/BF02638019
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DOI: https://doi.org/10.1007/BF02638019