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Rapid ethanolysis procedure for jojoba wax analysis by gas liquid chromatography

  • Technical
  • Published:
Journal of the American Oil Chemists Society

Abstract

A rapid ethanolysis procedure for preparing jojoba wax for gas liquid chromatographic analysis is described. The wax esters are hydrolyzed by refluxing 4 drops of jojoba wax in 5% HCl in anhydrous ethanol in a test tube. The resulting fatty acid ethyl esters and fatty alcohols are separated and quantitated by a single gas liquid chromatographic run. Analysis of duplicate samples by this procedure gives essentially the same results as a procedure which requires 10 times more sample and reagents and considerably more time.

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References

  1. Daugherty, P.M., H.H. Sineath, and T.A. Wastler, Econ. Bot. 12:296 (1958).

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  2. Miwa, T.K., JAOCS 48:259 (1971).

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Authors and Affiliations

  1. Department of Plant Sciences, University of California, 92502, Riverside, California

    C. C. Duncan, D. M. Yermanos, J. Kumamoto & C. S. Levesque

Authors
  1. C. C. Duncan
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  2. D. M. Yermanos
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  3. J. Kumamoto
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  4. C. S. Levesque
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Duncan, C.C., Yermanos, D.M., Kumamoto, J. et al. Rapid ethanolysis procedure for jojoba wax analysis by gas liquid chromatography. J Am Oil Chem Soc 51, 534–536 (1974). https://doi.org/10.1007/BF02636023

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  • DOI: https://doi.org/10.1007/BF02636023

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