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Radiochlorine as a tracer in fat deposition

  • Technical
  • Published:
Journal of the American Oil Chemists Society

Abstract

Radiochlorine38 was evaluated and found to be suitable for use as a tracer in studying the deposition of fat in porcinepanniculus adiposus tissue. Its short half-life makes its preparation from natural chlorine simple and inexpensive, and also minimizes long-term contamination problems, and its energetic beta and gamma rays makes its radio-assay simple and rapid. For swine, the threshhold level for a statistical counting accuracy of 1% was found to be approx 8 × 10−2 mc/lb of live wt. The time required for the digestion, absorption, transport and deposition of statistically sufficient detectable radioactive material was 3 1/2 hr. The incorporation of radiochlorine38 into lipid molecules does not appear to alter their normal metabolic pathways.

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References

  1. Bloom, B., I. L. Chaikoff and W. O. Reinhardt, Am. J. Physiol.166, 451–455 (1951).

    PubMed  CAS  Google Scholar 

  2. Burch, G. E., S. A. Threefoot and C. T. Ray, J. Lab. Clin. Med.35, 331–347, (1950).

    CAS  Google Scholar 

  3. Care, A. D., Proc. Roy. Soc. Med.48, 827–833 (1955).

    PubMed  CAS  Google Scholar 

  4. Comar, C. L., “Radioisotopes in Biology and Agriculture,” McGraw-Hill, New York, 1955, p. 235–236.

    Google Scholar 

  5. Comar, C. L., Proc. Inter. Conf. on the Peaceful Uses of Atomic Energy,12, 245–251 (1955).

    Google Scholar 

  6. Comar, C. L., and R. H. Wasserman, Atomic Energy and Agriculture Symposium, Am. Assoc. Adv. Sci., Washington, 1957, p. 286–292.

  7. Cowie, D. B., L. B. Flexner and W. S. Wilde, Am. J. Physiol.158, 231–236 (1949).

    CAS  Google Scholar 

  8. Glasstone, S., “Sourcebook on Atomic Energy,” 2nd ed., D. Van Nostrand, New York, 1958, p. 517–518.

    Google Scholar 

  9. Hevesy, G., Biochem. J.17, 439–445 (1923).

    PubMed  CAS  Google Scholar 

  10. Hevesy, G., Enzymologia5, 138–157 (1938).

    CAS  Google Scholar 

  11. Hogan, C. A. M., Proc. Nat. Acad. Sci. (U.S.A.)37, 393–395 (1951).

    Article  ADS  Google Scholar 

  12. Johannesson, J. K., Anal. Chem.34, 1111–1113 (1962).

    Article  CAS  Google Scholar 

  13. Kamen, M. D., “Radioactive Tracers in Biology,” Academic Press, New York, 1947, p. 222–224.

    Google Scholar 

  14. Mead, J. F., and D. R. Howton, “Radioisotopic Studies of Fatty Acid Metabolism,” Pergamon Press, New York, 1960, p. 1–36.

    Google Scholar 

  15. Sink, J. D., J. L. Watkins, J. H. Ziegler and R. C. Miller, J. An. Sci.23, 121–125 (1964).

    CAS  Google Scholar 

  16. Sorensen, P., Anal. Chem.27, 388–390 (1955).

    Article  CAS  Google Scholar 

  17. Sorensen, P.,Ibid. 28, 1318–1320 (1956).

    Article  CAS  Google Scholar 

  18. Strajman, E., and N. Pace, Adv. Biol. Med. Phys.2, 193–241 (1951).

    Google Scholar 

  19. Threefoot, S. A., G. E. Burch and C. T. Ray, J. Lab. Clin. Med.42, 16–33 (1953).

    PubMed  CAS  Google Scholar 

  20. Trum, B. F., J. N. Snively, U. S. G. Kuhn and W. T. Carll, Radiation Res.11, 326–342 (1959).

    Google Scholar 

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Journal Series of The Pennsylvania Agricultural Experiment Station, Paper No. 2818.

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Sink, J.D., Watkins, J.L., Ziegler, J.H. et al. Radiochlorine as a tracer in fat deposition. J Am Oil Chem Soc 42, 435–437 (1965). https://doi.org/10.1007/BF02635586

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  • DOI: https://doi.org/10.1007/BF02635586

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