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Variation in culture, isoenzyme patterns and plastid DNA in the genusDaucus

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Summary

To identify markers for fusion and transformation studies, cell suspension cultures of four members of theDaucus genus were examined to determine differences in culture conditions, isoenzyme patterns, and plastid DNA. The four were:D. carota subsp.sativus cv. Danvers,D. carota subsp.gummifer, D. capillifolius, andD. pusillus. Under appropriate conditions, all four grew well as liquid cell suspension cultures and regenerated from protoplasts into plants. Enzyme activities of homoserine dehydrogenase (HSDH) and alcohol dehydrogenase from cell culture extracts were analyzed on electrophoretic gels. Although only one form of HSDH was present in eachDaucus line, the rate of migration of HSDH from cv. Danvers was different from that of the other cell lines. Multiple isoenzymic forms of ADH were present in eachDaucus cultivar.

Camparison of endonuclease restriction fragment patterns from plastid DNAs digested by BamHI revealed only small differences between plastid DNAs of cv. Danvers and subsp.Gummifer, whereas large differences were observed between cv. Danvers andD. pusillus plastid DNA patterns. No differences were found between cv. Danvers andD. capillifolius plastid DNA patterns when examined using eight different restriction enzymes. The data indicate that specific isoenzyme and organelle DNA restriction fragment patterns will be useful markers for precise identification of genomes of differentDaucus species in somatic hybridization experiments.

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References

  1. Widholm, J. M. Control of aromatic amino acid biosynthesis in cultured plant tissues: Effect of intermediates and aromatic amino acids on free levels. Physiol. Plant. 30: 13–18; 1974.

    Article  CAS  Google Scholar 

  2. Widholm, J. M. Cultured carrot cell mutants: 5-methyltryptophan-resistant trait carried from cell to plant and back. Plant Sci. Lett. 3: 323–330; 1974.

    Article  CAS  Google Scholar 

  3. Widholm, J. M. Selection and characterization of cultured carrot and tobacco cells resistant to lysine, methionine, and proline analogs. Can. J. Bot. 54: 1523–1529; 1976.

    CAS  Google Scholar 

  4. Harms, C. T.; Oertli, J. J.; Widholm, J. M. Characterization of amino acid analogue resistant somatic hybrid cell lines ofDaucus carota L. Z. Pflanzenphysiol. 106: 239–249; 1982.

    CAS  Google Scholar 

  5. Harms, C. T.; Potrykus, I.; Widholm, J. M. Complementation and dominant expression of amino acid analogue resistance markers in somatic hybrid clones fromDaucus carota after protoplast fusion. Z. Pflanzenphysiol. 101: 377–390; 1981.

    CAS  Google Scholar 

  6. Kameya, T.; Horn, M. E.; Widholm, J. M. Hybrid shoot formation from fusedDaucus carota andD. capillifolius protoplasts. Z. Pflanzenphysiol. 104: 459–466; 1981.

    CAS  Google Scholar 

  7. Dudits, D.; Hadlaczky, E.; Levi, E.; Fejer, O.; Haydu, Z.; Lazar, G. Somatic hybridization ofDaucus carota andD. capillifolius by protoplast fusion. Theor. Appl. Genet. 51: 127–132; 1977.

    Google Scholar 

  8. Small, E. A numerical taxonomic analysis of theDaucus carota complex. Can. J. Bot. 56: 248–276; 1978.

    Article  Google Scholar 

  9. Kung, S. D.; Zhu, T. S.; Chen, D. K.; Shen, G. F.; Sison, V. A.Nicotiana chloroplast genome II. Chloroplast DNA alteration. Mol. Gen. Genet. 183: 20–24; 1981.

    Article  CAS  Google Scholar 

  10. Kung, S. D.; Zhu, Y. S.; Shen, G. F. Nicotiana chloroplast genome III. Chloroplast DNA evolution. Theor. Appl. Genet. 61: 73–79; 1982.

    Article  CAS  Google Scholar 

  11. Rhodes, P. R.; Zhu, Y. S.; Kung, S. D. Nicotiana chloroplast genome I. Chloroplast DNA diversity. Mol. Gen. Genet. 182: 106–111; 1981.

    Article  CAS  Google Scholar 

  12. Vedel, F.; Lebacq, P.; Quetier, F. Cytoplasmic DNA variation and relationships in cereal genomes. Theor. Appl. Genet. 58: 219–224; 1980.

    Article  CAS  Google Scholar 

  13. Aviv, D.; Fluhr, R.; Edelman, M.; Galun, E. Progeny analysis of the interspecific somatic hybrids:Nicotiana tabacum (CMS)+Nicotiana sylvestris with respect to nuclear and chloroplast markers. Theor. Appl. Genet. 56: 145–150; 1980.

    Article  CAS  Google Scholar 

  14. Sidorov, V. A.; Menczel, L.; Nagy, F.; Maliga, P. Chloroplast transfer inNicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts. Planta 152: 341–345; 1981.

    Article  CAS  Google Scholar 

  15. Menczel, L.; Nagy, N.; Kiss, Zs. R.; Maliga, P. Streptomycin resistant and sensitive somatic hybrids ofNicotiana tabacum+Nicotiana knightiana: correlation of resistance toN. tabacum plastids. Theor. Appl. Genet. 59: 191–195; 1981.

    Article  CAS  Google Scholar 

  16. Kumar, A.; Cocking, E. C.; Bovenberg, W. A.; Kool, A. J. Restriction endonuclease analysis of chloroplast DNA in interspecies somatic hybrids ofPetunia. Theor. Appl. Genet. 62: 377–383; 1982.

    CAS  Google Scholar 

  17. Schiller, B.; Herrmann, R. G.; Melchers, G. Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids. Mol. Gen. Genet. 186: 453–459; 1982.

    Article  CAS  Google Scholar 

  18. McCollum, G. D. Interspecific hybridDaucus carota×D. capillifolius. Bot. Gaz. 136: 201–206; 1975.

    Article  Google Scholar 

  19. McCollum, G. D. Hybrids ofDaucus gingidium with cultivated carrots (D. carota subsp. sativus) andD. capillifolius. Bot. Gaz. 138: 56–63; 1977.

    Article  Google Scholar 

  20. Widholm, J. M. CulturedNicotiana tabacum cells with an altered anthranilate synthetase which is less sensitive to feedback inhibition. Biochim. Biophys. Acta 261: 52–58; 1972.

    PubMed  CAS  Google Scholar 

  21. Matthews, B. F.; Widholm, J. M. Regulation of lysine and threonine synthesis in carrot cell suspension cultures and whole carrot roots. Planta 141: 315–321; 1978.

    Article  CAS  Google Scholar 

  22. Matthews, B.; Dray, S.; Widholm, J.; Ostro, M. Liposome-mediated transfer of bacterial RNA into carrot protoplasts. Planta 145: 37–44; 1979.

    Article  CAS  Google Scholar 

  23. Dudits, D.; Kao, K. N.; Constable, F.; Gamborg, O. L. Embryogenesis and formation of tetraploid and hexaploid plants from carrot protoplasts. Can. J. Bot. 54: 1063–1067; 1976.

    CAS  Google Scholar 

  24. Davis, B. J. Disc electrophoresis II. Method and application to human serum proteins. Ann. NY Acad. Sci. 121: 404–427; 1964.

    Article  PubMed  CAS  Google Scholar 

  25. Matthews, B. F.; Gurman, A. W.; Bryan, J. K. Changes in enzyme regulation during growth of maize. I. Progressive desensitization of homoserine dehydrogenase during seedling growth. Plant Physiol. 55: 991–998; 1975.

    PubMed  CAS  Google Scholar 

  26. Freeling, M.; Schwartz, D. Genetic relationships between the multiple alcohol dehydrogenases of maize. Biochem. Genet. 8: 27–36; 1973.

    Article  PubMed  CAS  Google Scholar 

  27. Nishimura, M.; Beevers, H. Isolation of intact plastids from protoplasts of castor bean endosperm. Plant Physiol. 62: 40–43; 1978.

    Article  PubMed  CAS  Google Scholar 

  28. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  29. Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158; 1968.

    Article  PubMed  CAS  Google Scholar 

  30. Schwartz, D. The genetic control of alcohol dehydrogenase in maize: gene duplication and repression. Proc. Natl. Acad. Sci. USA 56: 1431–1436; 1966.

    Article  PubMed  CAS  Google Scholar 

  31. Sacks, M. M.; Freeling, M. Selective synthesis of alcohol dehydrogenase during anaerobic treatment of maize. Mol. Gen. Genet. 161: 111–115; 1978.

    Google Scholar 

  32. Sacks, M. M.; Freeling, M.; Okimoto, R. The anaerobic proteins of maize. Cell 20: 761–767; 1980.

    Article  Google Scholar 

  33. Chourey, P. S.; Widholm, J. Tissue specific alcohol dehydrogenase isozyme variation in carrot: whole plant versus in vitro cultured cells. In Vitro 16: 571–574; 1980.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Tissue Culture and Molecular Genetics Laboratory, U.S. Department of Agriculture, S&E, ARS, PPHI, 20705, Beltsville, Maryland

    Benjamin F. Matthews

  2. Department of Botany, Miami University, 45056, Oxford, Ohio

    Kenneth G. Wilson & Lorin R. DeBonte

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  1. Benjamin F. Matthews
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  2. Kenneth G. Wilson
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  3. Lorin R. DeBonte
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This research was supported by the U.S. Department of Agriculture under Agreement 59-2246-1-1-737-0.

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Matthews, B.F., Wilson, K.G. & DeBonte, L.R. Variation in culture, isoenzyme patterns and plastid DNA in the genusDaucus . In Vitro 20, 38–44 (1984). https://doi.org/10.1007/BF02633330

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  • DOI: https://doi.org/10.1007/BF02633330

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