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Genotype effects on proliferative embryogenesis and plant regeneration of soybean

  • Developmental Biology/Morphogenesis
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Summary

Proliferative somatic embryogenesis is a regeneration system suitable for mass propagation and genetic transformation of soybean [Glycine max (L.) Merr.]. The objective of this study was to examine genotypic effects on induction and maintenance of proliferative embryogenic cultures, and on yield, germination, and conversion of mature somatic embryos. Somatic embryos were induced from eight genotypes by explanting 100 immature cotyledons per genotype on induction medium. Differences in frequency of induction were observed among genotypes. However, this step was not limiting for plant regeneration because induction frequency in the least responding genotype was sufficient to initiate and maintain proliferative embryogenic cultures. Six genotypes selected for further study were used to initiate embryogenic cultures in liquid medium. Cultures were evaluated for propagation of globular-stage tissue in liquid medium, yield of cotyledon-stage somatic embryos on differentiation medium, and plant recovery of cotyledon-stage embryos. Genotypes also differed for weight and volume increase of embryogenic tissue in liquid cultures, for yield of cotyledon-stage embryos on differentiation medium, and for plant recovery from cotyledon-stage embryos. Rigorous selection for a proliferative culture phenotype consisting of nodular, compact, green spheres increased embryo yield over that of unselected cultures, but did not affect the relative ranking of genotypes. In summary, the genotypes used in this study differed at each stage of plant regeneration from proliferative embryogenic cultures, but genotypic effects were partially overcome by protocol modifications.

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References

  • Buchheim, J. A.; Colburn, S. M.; Ranch, J. P. Maturation of soybean somatic embryos and the transition to plantlet growth. Plant Physiol. 89:768–775; 1989.

    PubMed  CAS  Google Scholar 

  • Finer, J. J. Apical proliferation of embryogenic tissue of soybean [(Glycine max (L.) Merrill]. Plant Cell Rep. 7:238–241; 1988.

    Article  Google Scholar 

  • Finer, J. J.; McMullen, M. D. Transformation of soybean via particle bombardment of embryogenic suspension culture tissue. In Vitro Cell. Dev. Biol. 27P:175–182; 1991.

    CAS  Google Scholar 

  • Finer, J. J.; Nagasawa, A. Development of an embryogenic suspension culture of soybean (Glycine max Merrill.). Plant Cell Tissue Organ Cult. 15:125–136; 1988.

    Article  CAS  Google Scholar 

  • Fujii, J. A. A.; Slade, D.; Redenbaugh, K. Maturation and greenhouse planting of alfalfa artificial seeds. In Vitro Cell. Dev. Biol. 25:1179–1182; 1989.

    Article  Google Scholar 

  • Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:150–158; 1968.

    Article  Google Scholar 

  • Hammatt, N.; Davey, M. R. Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean (Glycine max L. Merr.). J. Plant Physiol. 128:219–226; 1987.

    Google Scholar 

  • Hartmann, H. T.; Kester, D. E. Principles of propagation by seeds. In: Plant propagation: principles and practices. Englewood Cliffs, NJ: Prentice-Hall, Inc.; 1975:108–145.

    Google Scholar 

  • Hughes, D. W.; Galau, G. A. Temporally modular gene expression during cotyledon development. Genes Dev. 3:358–369; 1989.

    PubMed  CAS  Google Scholar 

  • Kermode, A. R. Regulatory mechanisms involved in the transition from seed development to germination. Crit. Rev. Plant Sci. 9:155–195; 1990.

    Article  CAS  Google Scholar 

  • Komatsuda, T. Ability of soybean (Glycine max L. Merr.) genotypes to produce somatic embryos on a medium containing a low concentration of sucrose. Jpn. J. Breed. 40:371–375; 1990.

    Google Scholar 

  • Komatsuda, T.; Kaneko, K.; Oka, S. Genotype x sucrose interactions for somatic embryogenesis in soybean. Crop Sci. 31:333–337; 1991.

    Article  CAS  Google Scholar 

  • Komatsuda, T.; Lee, W.; Oka, S. Maturation and germination of somatic embryos as affected by sucrose and plant growth regulators in soybeansGlycine gracilis Skvortz andGlycine max (L.) Merr. Plant Cell Tissue Organ Cult. 28:103–113; 1992.

    Article  CAS  Google Scholar 

  • Komatsuda, T.; Ko, S-W. Screening of soybean [Glycine max (L.) Merrill] genotypes for somatic embryo production from immature embryo. Jpn. J. Breed. 40:371–375; 1990.

    Google Scholar 

  • Komatsuda, T.; Ohyama, K. Genotypes of high competence for somatic embryogenesis and plant regeneration in soybeanGlycine max. Theor. Appl. Gen. 75:695–700; 1988.

    Article  Google Scholar 

  • Lazzeri, P. A.; Hildebrand, D. F.; Collins, G. B. A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol. Biol. Rep. 3:160–167; 1985.

    Google Scholar 

  • Lippmann, B.; Lippmann, G. Induction of somatic embryos in cotyledonary tissue of soybean,Glycine max L. Merr. Plant Cell Rep. 3:215–218; 1984.

    Article  CAS  Google Scholar 

  • Liu, W.; Moore, P. J.; Collins, G. B. Somatic embryogenesis in soybean via somatic embryo cycling. In Vitro Cell. Dev. Biol. 28P:153–160; 1992.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Parrott, W. A.; Dryden, G.; Vogt, S., et al. Optimization of somatic embryo-genesis and embryo germination in soybean. In Vitro Cell. Dev. Biol. 24:817–820; 1988.

    Article  CAS  Google Scholar 

  • Parrott, W. A.; Williams, E. G.; Hildebrand, D. F., et al. Effect of genotype on somatic embryogenesis from immature cotyledons of soybean. Plant Cell Tissue Organ Cult. 16:15–21; 1989.

    Article  Google Scholar 

  • Ranch, J. P.; Oglesby, L.; Zielinski, A. C. Plant regeneration from embryo-derived tissue cultures of soybeans. In Vitro Cell. Dev. Biol. 21:653–658; 1985.

    Article  Google Scholar 

  • Shoemaker, R. C.; Amberger, L. A.; Palmer, R. G., et al. Effect of 2,4-dichlorophenoxyacetic acid concentration on somatic embryogenesis and heritable variation in soybean [Glycine max (L.) Merr.]. In Vitro Cell. Dev. Biol. 27P:84–88; 1991.

    CAS  Google Scholar 

  • Wright, M. S.; Launis, K. L.; Novitzky, R., et al. A simple method for the recovery of multiple fertile plants from individual somatic embryos of soybean [Glycine max (L.) Merrill]. In Vitro Cell. Dev. Biol. 27P:153–157; 1991.

    Google Scholar 

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Authors and Affiliations

  1. Department of Crop and Soil Sciences, The University of Georgia, 30602, Athens, Georgia

    M. A. Bailey, H. R. Boerma & W. A. Parrott

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  1. M. A. Bailey
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  2. H. R. Boerma
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  3. W. A. Parrott
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Bailey, M.A., Boerma, H.R. & Parrott, W.A. Genotype effects on proliferative embryogenesis and plant regeneration of soybean. In Vitro Cell Dev Biol - Plant 29, 102–108 (1993). https://doi.org/10.1007/BF02632279

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  • DOI: https://doi.org/10.1007/BF02632279

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