Skip to main content
SpringerLink
Log in

Modulation of somatic embryogenesis in hypocotyl-derived cultures of geranium (Pelargonium X hortorum bailey) CV ringo rose by a bacterium

  • Developmental Biology/Morphogenesis
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Summary

The Ringo Rose cultivar of zonal geranium (Pelargonium x hortorum Bailey) has been shown to be morphogenetically unresponsive. Attempts to improve somatic embryogenesis using various seed stress treatments before germination proved ineffective. However, bacterial contamination of one of the seed-stress treatments led to infected explants that had a significant increase in frequency of high-quality somatic embryos. The co-cultivation of explants with the isolated bacterium (tentatively identified asBacillus sp.) was found to be repeatable, and potentially represents a novel way to improve morphogenesis in geranium and possibly other species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Agrios, G. N. Plant pathology. San Diego, CA: Academic Press; 1988:79–86.

    Google Scholar 

  • Barwale, U. B.; Kerns, H. R.; Widholm, J. M. Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167:473–481; 1988.

    Article  Google Scholar 

  • Brown, D. C. W. Germplast determination ofin vitro somatic embryogenesis in alfalfa. HortScience 23:526–531; 1988.

    Google Scholar 

  • Cassells, A. C. The effect of 2,3,5-triiodobenzoic acid on caulogenesis in callus cultures of tomato andPelargonium. Physiol. Plant. 46:159–164; 1979.

    Article  CAS  Google Scholar 

  • Dunbar, K. B.; Stephens, C. T. Shoot regeneration of hybrid seed geranium (Pelargonium x hortorum) and regal geranium (Pelargonium x domesticum) from primary callus cultures. Plant Cell Tissue Organ Cult. 19:13–21; 1989.

    Article  Google Scholar 

  • Gill, R.; Gerrath, J.; Saxena, P. K. High-frequency direct somatic embryogenesis in thin layer cultures of hybrid seed geranium. Can. J. Bot. 71:408–413; 1993.

    Google Scholar 

  • Gill, R.; Senaratna, T.; Saxena, P. K. Thidiazuron-induced somatic embryogenesis enhances viability of hydrogel-encapsulated somatic embryos of geranium. J. Plant Physiol (in press); 1994.

  • Hodges, T. K.; Kamo, K. K.; Imbrie, C. W., et al. Genotype specificity of somatic embryogenesis and regeneration in maize. Bio/Technology 4:219–223; 1986.

    Article  Google Scholar 

  • Keyes, G. J.; Collins, B. G.; Taylor, L. N. Genetic variation in tissue cultures of red clover. Theor. Appl. Genet. 58:265–271; 1980.

    Article  Google Scholar 

  • Kiyosue, T.; Takano, K.; Kamada, H., et al. Induction of somatic embryogenesis in carrot by heavy metal ions. Can. J. Bot. 68:2301–2303; 1990.

    CAS  Google Scholar 

  • Marsolais, A. A.; Wilson, D. P. M.; Tsujita, M. J.; Senaratna, T. Somatic embryogenesis and artificial seed production in Zonal (Pelargonium ×hortorum) and Regal (Pelargonium ×domesticum) geranium. Can. J. Bot. 169:1188–1193; 1991.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–479; 1962.

    Article  CAS  Google Scholar 

  • Ou, G.; Wang, W. C.; Nguyen, H. T. Inheritance of somatic embryogenesis and organ regeneration from immature embryo cultures of winter wheat. Theor. Appl. Genet. 78:137–142; 1989.

    Article  Google Scholar 

  • Pillai, S. K.; Hildebrandt, A. C. Geranium plants differentiated in vitro from stem tip and callus cultures. Plant Dis. Rep. 52:600–601; 1968.

    Google Scholar 

  • Redenbaugh, K.; Slade, D.; Viss, P., et al. Encapsulation of somatic embryos in synthetic seed coats. HortScience 22:803–809; 1987.

    Google Scholar 

  • Slimmon, T.; Qureshi, J. A.; Saxena, P. K. Phenylacetic acid induced somatic embryogenesis in cultured hypocotyl explants of geranium (Pelargonium x hortorum Baily). Plant Cell Rep. 10:587–589; 1991.

    Article  CAS  Google Scholar 

  • Theiler, R.In vitro culture of shoot tips ofPelargonium species. Acta Hortic. 78:403–409; 1977.

    Google Scholar 

  • Thorpe, T. A.In vitro somatic embryogenesis. ISI Atlas Sci. Anim. Plant Sci. 1:81–88; 1988.

    Google Scholar 

  • Visser, C.; Qureshi, J. A.; Gill, R., et al. Morphoregulatory role of thidiazuron: substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol. 99:1704–1707; 1992.

    Article  PubMed  CAS  Google Scholar 

  • Wake, H.; Umetsu, H.; Ozeki, Y.; et al. Extracts of marine cyanobacteria stimulated somatic embryogenesis ofDaucus carota L. Plant Cell Rep. 9:655–658; 1991.

    Article  Google Scholar 

  • Wake, H.; Akasaka, A.; Umetsu, H., et al. Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium. Plant Cell Rep. 11:62–65; 1992.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Christena Visser-Tenyenhuis, B. N. S. Murthy & Praveen K. Saxena

  2. Ontario Ministry of Agriculture and Food, N1H 8J7, Guelph, Ontario, Canada

    Joseph Odumeru

Authors
  1. Christena Visser-Tenyenhuis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. N. S. Murthy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joseph Odumeru
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Praveen K. Saxena
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Visser-Tenyenhuis, C., Murthy, B.N.S., Odumeru, J. et al. Modulation of somatic embryogenesis in hypocotyl-derived cultures of geranium (Pelargonium X hortorum bailey) CV ringo rose by a bacterium. In Vitro Cell Dev Biol - Plant 30, 140–143 (1994). https://doi.org/10.1007/BF02632203

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02632203

Key words

Search

Navigation