Summary
An experimental rooting system was developed to study in vitro adventitious root formation in hazelnut cotyledons. Experiments involveda) assay of several culture media,b) use of different developmental status of the seeds (germinated and ungerminated),c) cotyledons subjected to various light regimes, andd) different size of cotyledons slices. It was observed that higher rooting was induced in cotyledonary portions of 5- or 7-mm thickness (250 and 350 mm3, respectively) cultured on half-strength basal medium supplemented with 50 µM indole-3-butyric acid and 5 µM kinetin. Rooting was affected by light and the developmental state of seeds. Preinitiation, initiation, and root manifestation stages were defined according to specific culture periods and in relation with morphologic and histologic changes. The first histologic changes (cell division and root primordia induction) were observed after 12 days in culture. At 30 days of culture in rhizogenic medium root primodia were fully differentiated with well-developed vascular tissues.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amerson, H. V.; Frampton, L. J., Jr.; McKeand, S. E., et al. Loblolly pine tissue culture: laboratory, greenhouse, and field studies. In: Henke, R. R., ed. Tissue culture in forestry and agriculture. New York: Plenum Press; 1985:271–287.
Batten, D.; Goodwin, P. B. Phytohormones and related compounds—a comprehensive treatise. In: Lethan, D. S.; Goodwin, P. B.; Higgins, T. J., eds. Phytohormones and the development of plants, 2. New York: Elsevier/NorthHolland Biomedical Press; 1978:137–173.
Coleman, W. K.; Greyson, R. I. Analysis of root formation in leaf discs ofLicopersicon esculentum Mill. cultured in vitro. Ann. Bot. 41:307–320; 1977.
Cheng, T. Y. Adventitious bud formation in culture of Douglas fir (Pseudotsuga mensziensii Mirb. Franco). Plant Sci. Lett. 5:97–102; 1975.
Eliasson, L. Growth regulators inPopulus tremula. Effect of light on inhibitor content in root suckers. Physiol. Plant. 24:205–208; 1971.
Eliasson, L. Interaction of light and auxin in regulation of rooting in pea stem cuttings. Physiol. Plant. 48:78–82; 1980.
Eliasson, L. Factors affecting the inhibitory effect of indolylacetic acid on root formation in pea cuttings. Physiol. Plant. 51:23–26; 1981.
Eliasson, L.; Bollmark, M. Ethylene as a possible mediator of light induced inhibition of root growth. Physiol. Plant. 72:605–609; 1988.
Eliasson, L.; Brunes, L. Light effects on root formation in aspen and willow cuttings. Physiol. Plant. 48:78–82; 1980.
Fabijan, D.; Yeung, E.; Mukherjee, I., et al. Adventitious rooting in hypocotyls of sunflower (Heliantus annuus) seedlings I. Correlative influences and developmental sequence. Physiol. Plant. 53:578–588; 1981.
Feder, N.; O’Brien, T. P. Plant microthecnique: some principles and new methods. Am. J. Bot. 55:123–127; 1968.
Feldman, L. J. Regulation of root development. Annu. Rev. Plant Physiol. 35:223–242; 1984.
Feldman, L.; Arroyave, N.; Sun, P. S. Abscisic acid, xanthoxin and violaxanthin in the caps of gravistimulated maize roots. Planta 166:483–489; 1985.
Gaspar, T.; Coumans, M. Root formation. In: Bonga, J. M., Durzan, D. J., eds. Cell and tissue culture in forestry 3. Boston: Martinus Nijhoff Publishers. 1987:202–217.
Gautheret, R. J. Actión conjuguéede l’acide gibberellique de la cinetine et de l’acide indole-acetique sue les tissus cultives “in vitro”, particulierement sur ceux de Topinambur. C. R. Acad. Sci. Paris 253:1381–1385; 1961.
Gautheret, R. J. Cell differentiation and morphogenesis. Amsterdam: North-Holland Publishers; 1966:54–95.
González, A.; Rodríguez, R.; Sánchez Tamés, R. Ethylene and in vitro rooting on (Corylus avellana L) cotyeldons. Physiol. Plant. 81:227–233; 1991.
Guenther, W. E. Analysis of variance. Englewood Cliffs: Prentice-Hall.
Hackett, W. P. The influence of auxin, catechol and methanolic extracts on root initiation in aseptically cultured shoot apices of the juvenile and mature forms ofHedera helix. J. Am. Soc. Hortic. Sci. 95:398–402; 1970.
Haissig, B. E. Metabolic processes in adventitious rooting of cuttings. In: Jackson, M. B., ed. New root formation in plant and cuttings. Boston: Martinus Nijhoff Publishers, 1986:142–189.
Haissig, B. E. Future directions in adventitious rooting research. In: Davis, T. D.; Haissig, B. E.; Sankhla, N., eds. Adventitious root formation in cutting. Advances in Plant Sciences Series, 2. Portland, Oregion: Dioscorides Press; 1988:302–310.
Hartmann, H. T.; Kester, D. E. Plant propagation: principles and practices, 4th ed. Englewood Cliffs, NJ: Prentice Hall; 1983.
Johansen, D. A. Plant microtechnique. New York: McGraw-Hill; 1940.
Maene, L.; Debergh, P. Rooting of tissue cultured plants under in vivo conditions. Acta Hortic. 131:201–208; 1983.
Markland, W.; Haddon, L. An improved technique for the culture of Jerusalen artichoke (Helianthus tuberosus L) explants for use in the study of xylem differentiation. Plant Cell Rep. 1:229–231; 1982.
Masuda, Y. Effect of light on a growth inhibitor in wheat roots. Physiol. Plant. 15:780–790; 1962.
McCown, B. H. Adventitious rooting of tissue cultured plants. In: Davis, T. D.; Haissig, B. E.; Sankhla, N., eds. Adventitious root formation in cuttings. Advances in Plant Sciences Series, 2. Portland, Oregon: Discorides Press; 1988:289–302.
Mullins, M. G. Regulation of adventitious root formation in microcuttings. Acta Hortic. 166:53–61; 1985.
Murashige, T. Plant propagation through tissue cultures. Ann. Rev. Plant Physiol. 25:135–166; 1974.
Nougarede, A.; Rondet, P. Bases cytophysiologiques de l’induction rhizogene en reponse a un traitement auxinique dans l’epicotyle du pois nain. Ann. Sci. Nat. Bot. Paris 5:121–149; 1983.
Pilet, P.; Rivier, L. Light and dark georeaction of maize roots: effect and endogenous level of abscisic acid. Plant Sci. Lett. 18:201–206; 1980.
Quoring, M. Premiers resultats obtenus dans la culture “in vitro” du meristeme apical de sujets portegreffe de pomier. Bull. Rech. Agron, Gembloux 9:189–192; 1974.
Rajagopal, V.; Andersen, A. S. Water stress and root formation in root cuttings I. Influence of the degree and duration of water stress on stock plants grown under two levels of irradiance. Physiol. Plant. 48:144–149; 1980.
Rodríguez, R.; Rodríguez, A.; González, A., et al. Hazelnut (Corylus avellana L). In: Bajaj, Y., ed. Biotechnology in agriculture and forestry 5. Berlin: Springer-Verlag; 1989:127–160.
Sangwan, R. S.; Harada, H. Chemical regulation of callus growth, organogenesis and plant regeneration inAnthirrinum majus tissue and cell cultures. J. Exp. Bot. 26:868–882; 1975.
Thorpe, T.; Patel, K. R. Clonal propagation: adventitious buds. In: Vasil, I. K., ed. Cell culture and somatic cell genetic of plant 1. New York: Academic Press; 1984:49–60.
Veierskov, B.; Hansen, J.; Andersen, A. S. Influence of cotyledon excision and sucrose on root formation in pea cuttings. Physiol. Plant. 36:105–109; 1976.
Zimmerman, R. H.; Griesbach, R. J.; Hammerschlag, F. A., et al. Tissue Culture as a Plant Production System for Horticultural Crops. Norwell, MA: Kluwer-Nijhoff Publishing; 1986.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
González, A., Casares, A., Sánchez, T.R. et al. Adventitious root induction inCorylus avellana L. cotyledon slices. In Vitro Cell Dev Biol - Plant 27, 125–131 (1991). https://doi.org/10.1007/BF02632195
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02632195