Summary
The uptake of nitrate and ammonium by callus ofPlatycerium coronarium from the culture medium was examined. Nitrate reductase activity of photoautotrophic callus cultures under CO2 enrichment was significantly lower compared to the cultures without CO2 enrichment, but higher than that of heterotrophic callus cultured on medium with 2% (wt/vol) sucrose. When sucrose concentration of the heterotrophic culture was lowered to 0.2%, nitrate reductase activity increased. The level of nitrate reductase activity increased by about 25% in the heterotrophic callus with an increase in 2,4-D from 2 µM to 10 µM, despite a decline in fresh weight gain. However, photoautotrophic cultures with 1% CO2 enrichment showed 20% decline in nitrate reductase activity and 45% decline in fresh weight gain with a similar increase in 2,4-D level. The rate of uptake of nitrate from the culture medium was unrelated to the level of nitrate reductase activity in the callus. For photoautotrophic callus under CO2 enrichment, the presence of 1% (vol/vol) CO2 generally resulted in the highest rate of nitrate uptake. The rate of uptake of ammonium was higher for callus cultured on 2 µM 2,4-D compared to that on 10 µM 2,4-D.
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Alfonso, de C.; Angel, de la T.; Begona, D., et al. Role of light and CO2 fixation in the control of nitrate-reductase activity in barley leaves. Planta 190:277–283; 1993.
Bender, L.; Kumar, A.; Neumann, K. H. On the photosynthetic system and assimilate metabolism of Daucus and Arachis cell cultures. In: Neumann, K. H.; Barz, W.; Reinhard, E., eds. Primary and secondary metabolism of plant cell cultures. Berlin, Germany: Springer-Verlag; 1985:26–42.
Betsche, T. Atmospheric CO2 enrichment: kinetics of chlorophyll a fluorescence and photosynthetic CO2 uptake in individual, attached cotton leaves. Environ. Exp. Bot. 34:75–86; 1994.
Bowes, G. Growth at elevated CO2: photosynthetic responses mediated through Rubisco. Plant Cell Environ. 14:795–806; 1991.
Breteler, H.; Siegerist, M. Effect of ammonium on nitrate utilisation by roots of dwarf bean. Plant Physiol. 75:1099–1103; 1984.
Cawse, P. A. The determination of nitrate in soil solutions by ultraviolet spectrophotometry. Analyst 92:311–315; 1967.
Doddema, H.; Hofstra, J. J.; Feenstra, W. J. Uptake of nitrate by mutants ofArabidopsis thaliana, disturbed in uptake of nitrate and chlorate. Physiol. Plant. 43:343–350; 1978.
Hanisch ten Cate, C. H.; Bretelar, H. Role of sugars in nitrate utilisation by roots of dwarf bean. Physiol. Plant. 52:129–135; 1981.
Hecht, U.; Mohr, H. Factors controlling nitrate and ammonium accumulation in mustard (Sinapis alba) seedlings. Physiol. Plant. 78:379–387; 1990.
Hocking, P. J.; Meyer, C. P. Effects of CO2 enrichment and nitrogen stress on growth and partitioning of dry matter and nitrogen in wheat and maize. Aust. J. Plant Physiol. 18:339–396; 1991.
Kaiser, W. M.; Spill, D.; Brendle-Behnisch, E. Adenine nucleotides are apparently involved in the light-dark modulation of spinach leaf nitrate reductase. Planta 186:236–240; 1992.
Kwa, S. H.; Wee, Y. C.; Lim, T. M., et al. Establishment and physiological analyses of photoautotrophic callus cultures of the fernPlatycerium coronarium (Koenig) Desv. under CO2 enrichment. J. Exp. Bot.; in press: 1995.
Lillo, C. Light regulation of nitrate reductase in green leaves of higher plants. Physiol. Plant. 90:616–620; 1994.
Lowry, O. H.; Rosebrough, N. J.; Farr, A. L., et al. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275; 1951.
MacKnown, C. T.; Volk, R. J.; Jackson, W. A. Nitrate assimilation by decapitated corn root systems: effects of ammonium during induction. Plant Sci. Lett. 24:295–302; 1982.
Michel, V.; Therese, M.; Marie-Therese, L., et al. Regulation of nitrate and nitrite reductase expression inNicotiana plumbaginifolia leaves by nitrogen and carbon metabolites. The Plant J. 3(2):315–324; 1993.
Mohanty, B.; Fletcher, J. S. Ammonium influence on the growth and nitrate reductase activity of Paul’s Scarlet rose suspension cultures. Plant Physiol. 58:152–155; 1976.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 5:473–497; 1962.
Oaks, A. Efficiency of nitrogen utilisation in C3 and C4 cereals. Plant Physiol. 106:407–414; 1994.
Robinson, J. M. Spinach leaf chloroplast CO2 and NO 2− photoassimilations do not compete for photoregenerated reductant. Manipulation of reductant levels by quantum flux density titrations. Plant Physiol. 88:1373–1380; 1988.
Rogers, S. M. D.; Widholm, J. M. Photosynthetic characteristics of photoautotrophic cell suspensions of soybean. Plant Physiol. 80:S-46; 1986.
Subbaiah, C. C.; Balasimha, D. Nitrate reductase activity during ontogeny of the fruit of cashew (Anacardium occidentale). Aust. J. Plant Physiol. 10(1):9–14; 1983.
Yamada, Y.; Sato, F. The photoautotrophic culture of chlorophyllous cells. Plant Cell Physiol. 19:691–699; 1978.
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Kwa, SH., Wee, YC. & Kumar, P.P. Ammonium and nitrate uptake and nitrate reductase activity of photoautotrophic callus cultures of the fernPlatycerium coronarium (Koenig) DESV. In Vitro Cell Dev Biol - Plant 31, 211–214 (1995). https://doi.org/10.1007/BF02632024
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DOI: https://doi.org/10.1007/BF02632024