Summary
Satellite cells, liberated from pectoral muscle of juvenile dystrophic chickens by sequential treatment with collagenase, hyaluronidase, and trypsin and preplated to remove fibroblasts and cultured on gelatin proliferated rapidly, fused and formed confluent muscle cultures within 6 d in vitro with minimal contamination by fibroblasts. When identical isolation and culturing techniques were applied to muscle from age-mateched normal chickens proliferation and differentiation were slower, contamination with fibroblasts was much greater, and only a small number of myotubes were formed. After injection of the myotoxic anesthetic marcaine into normal pectoral muscle for 5 consecutive days, myotube formation was accelerated in satellite cell cultures, but the rate of differentiation was not as rapid as that occurring in cells from dystrophic muscle.
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Harris, J. B. Muscular dystrophy and other inherited diseases of skeletal muscle in animals. Harris, J. B. ed. Ann. NY Acad. Sci. Vol. 317. New York, NY: The New York Academy of Sciences; 1979.
Askanas, V.; Engel, W. K.; Multidimensional studies of diseased human cultured muscle seeking the pathogenesis of neuromuscular disorders. Den Hartos Jaser, W. A. et al. eds. Amsterdam: Neurology, Excerpta Medica; 1978: 93–107.
Kagawa, T.; Chikata, T.; Tani, J.; Tsutamune, T. In vitro myogenesis of the mono-nucleate cells derived from regenerating muscles of adult dystrophic mice. Dev. Biol. 65: 526–530; 1978.
Yasin, R.; Van Beers, G.; Bullien, D.; Thompson, E. J. A quantitative procedure for the dissociation of adult muscle into mononucleated cells. Exp. Cell. Res. 102: 405–407; 1976.
Cossu, G.; Zani, B.; Coletts, M.; Bouche, M.; Pacifici, M.; Molinaro, M.; In vitro differentiation of satellite cells isolated from normal and dystrophic mammalian muscles. A comparison with embryonic myogenic cells. Cell Differ. 9: 357–368; 1980.
Mauro, A. Satellite cell of skeletal muscle fibers. J. Biophys. Biochem. Cytol. 9: 493–495; 1961.
Bischoff, R. Enzymatic liberation of myogenic cells from adult rat muscle. Anat. Rec. 180: 645–662; 1974.
Asmundson, Y. S.; Julien, L. M.; Inherited muscle abnormality in the domestic fowl. J. Hered. 47: 248–252; 1956.
Wilson, B. W.; Randall, W. R.; Patterson, G. T.; Entrikin, R. K. Major physiologic and histochemical characteristics of inherited dystrophy in the chicken. Harris, J. B. ed. Muscular dystrophy and other inherited diseases of skeletal muscle in animals. Ann. N.Y. Acad. Sci. 317: 224–246; 1979.
Paul, J.; Cell and tissue culture, 2nd ed. London: E. S. Livingstone; 1981.
Hall-Craggs, E. C. B. Early ultrastructural changes in skeletal muscle exposed to the local anesthetic bupivicaine (Marcaine). Br. J. Exp. Pathol. 61: 139–149; 1980.
Nichols, J. R.; Shafiq, S. A. Muscle regeneration in the muscular dystrophes. Ann. NY Acad. Sci. 317: 478–493; 1979.
Yorita, T.; Nakamura, H.; Nonaka, I. Satellite cells and muscle regeneration in the developing dystrophic chickens. Exp. Neurol. 70: 567–575; 1980.
Jones, P. H. Isolation and culture of myogenic cells from regenerating rat skeletal muscle. Exp. Cell. Res. 107; 111–117; 1977.
Summers, P. J.; Parsons, R.; A quantitative assessment of dystrophic mouse (129 ReJ dy/dy) myogenesis in vitro. Neuropath. Appl. Neurobiol. 7: 269–277; 1981.
Askanas, V.; Shafiq, S. A.; Milhorat, A. T. Normal and dystrophic chicken muscle at success stages in tissue culture. Arch. Neurol. 24: 259–265; 1971.
Allan, E. R.; May, J. F.; Aspects of normal and dystrophic chicken muscle grown in vitro. Virchows Arch. [Cell Pathol.] 31: 243–250; 1979.
Powell, J. A. Development of normal and genetically dystrophic mouse muscle in tissue culture. Exp. Cell. Res. 80: 251–264; 1973.
Gieger, R. S., Garvin, J. S. Pattern of regeneration of muscle from progressive muscular dystrophy patients cultivated in vitro as compared to normal human skeletal muscle. J. Neuropathol. Exp. Neurol. 16: 532–543; 1957.
Herrmann, H.; Konigsberg, V. R.; Robinson, G. Observations on culture in vitro of normal and dystrophic muscle tissue. Proc. Soc. Exp. Biol. Med. 105: 217–221; 1960.
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This research was supported by a grant from the Muscular Dystrophy Association of Canada.
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Johnson, D.D., Wilcox, R. & Wenger, B. Precocious in vitro development of satellite cells from dystrophic chicken muscle. In Vitro 19, 723–729 (1983). https://doi.org/10.1007/BF02628964
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DOI: https://doi.org/10.1007/BF02628964