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Further purification of a fibroblast growth factor-like factor from chick embryo extract by heparin-affinity chromatography

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Summary

A mitogenic factor which promotes quail myoblast proliferation has been purified some 105-fold from chick embryo extract by a combination of cation-exchange chromatography and heparin-affinity chromatography. The factor is eluted from heparin-Sepharose with 2M NaCl and is a single-chain polypeptide with an apparent molecular weight of 15000 to 17000. It is active at subnanogram level in triggering the proliferation and thereby delaying temporarily fusion of myoblasts. It also stimulates the proliferation of quail fibroblasts in a similar effective concentration range. For both myoblasts and fibroblasts the dose-response to the factor is quantitatively and qualitatively comparable with that of bovine pituitary fibroblast growth factor. These observations strongly suggest that the factor very probably corresponds to chicken fibroblast growth factor or to a closely related molecule(s) and that it is possibly involved in the regulation of myogenesis.

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Author notes

  1. Ichiro Kimura

    Present address: Department of Basic Human Sciences, School of Human Sciences, Waseda University, Tokorozawa, 359, Saitama, Japan

Authors and Affiliations

  1. Division of Cell Biology, National Institute of Neuroscience, National Center for Neurology and Psychiatry, Kodaira, 187, Tokyo, Japan

    Ichiro Kimura, Yae Gotoh & Eijiro Ozawa

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  1. Ichiro Kimura
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  2. Yae Gotoh
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Additional information

This work was partly supported by a grant from the National Center of Neurology and Psychiatry (NCNP grant 86-01) of the Ministry of Health and Welfare, Japan.

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Kimura, I., Gotoh, Y. & Ozawa, E. Further purification of a fibroblast growth factor-like factor from chick embryo extract by heparin-affinity chromatography. In Vitro Cell Dev Biol 25, 236–242 (1989). https://doi.org/10.1007/BF02628460

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