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Growth of rat mammary tumor line 64-24 in liposome-supplemented defined medium. I. Effect of liposome B components on colony growth

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Summary

An improved serum-free medium has been developed that supports growth of rat mammary tumor line 64–24 with far less protein supplementation and with a much smaller inoculum than previously possible. An initial survey showed that MCDB 202 supported clonal growth with 1% dialyzed serum. The remaining serum was then replaced with 5 μg/ml insulin, 10 ng/ml epidermal growth factor (EGF), 1 μg/ml hydrocortisone, 50 ng/ml ovine prolactin, and 5 μg/ml liposome B (a mixture of soy lecithin, sphingomyelin, cholesterol, vitamin E, and vitamin E acetate in liposome form). Insulin and EGF are required and growth is improved by hydrocortisone and prolactin. Estradiol is stimulatory in the absence of liposome B. With adequate iron supplementation, transferrin has no effect. Liposome B increases growth rate substantially. Most of the growth stimulation can be replaced with phosphatidylethanolamine or sphingomyelin.

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Author notes

  1. Brigitte A. van der Haegen

    Present address: Department de Morfologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Sergipe, 49100, São Cristovão, SE, Brasil

Authors and Affiliations

  1. Department of Molecular, Cellular and Developmental Biology, University of Colorado, Campus Box 347, 80309, Boulder, Colorado

    Brigitte A. van der Haegen & Richard G. Ham

  2. Department de Morfologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Sergipe, 49100, São Cristovão, SE, Brasil

    Tamiko Kano-Sueoka

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  1. Brigitte A. van der Haegen
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  2. Richard G. Ham
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  3. Tamiko Kano-Sueoka
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This paper contains material from a thesis submitted to the Graduate School of the University of Colorado, Boulder, by B.A.VdH. in partial fulfillment of the requirements for the Ph.D. degree. This research was supported by grant CA-15305 to R.G.H. and grant CA-30545 to T.K.-S., both from the National Institutes of Health, Bethesda, MD.

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van der Haegen, B.A., Ham, R.G. & Kano-Sueoka, T. Growth of rat mammary tumor line 64-24 in liposome-supplemented defined medium. I. Effect of liposome B components on colony growth. In Vitro Cell Dev Biol 25, 151–157 (1989). https://doi.org/10.1007/BF02626172

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