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Isolation and characterization of an adriamycin-resistant breast tumor cell line

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Summary

An adriamycin-resistant human breast tumor cell line MDA-A1R was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1R cells grow as loosely attached cell aggregates with a doubling time of 28–32 h; the MDA-MB-231 parent cell line grows as a standard monolayer culture with a 20-h doubling time. The MDA-A1R cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine suggestive of a multidrug resistance (MDR) phenotype. MDA-A1R cells exhibit reduced net adriamycin conent as compared to the parent cell line. The MDR-associated P-glycoprotein gene is amplified approximately 10-to 30-fold in MDA-A1R cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal. Moreover, MDA-A1R cells show the presence of very high levels of P-glycoprotein. MDA-A1R is thus an in vitro model system to study the mechanism of MDR in human breast cancer.

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Author notes

  1. William L. Mc Guire

    Present address: Department of Medicine Divisions of Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284, San Antonio, Texas

Authors and Affiliations

  1. Department of Medicine Divisions of Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284, San Antonio, Texas

    Sandra L. Schneider, Suzanne A. W. Fuqua & Atul K. Tandon

  2. Gastronenterology/Nutrition, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284, San Antonio, Texas

    Kermit V. Speeg

Authors
  1. Sandra L. Schneider
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  2. Suzanne A. W. Fuqua
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  3. Kermit V. Speeg
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  4. Atul K. Tandon
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  5. William L. Mc Guire
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Additional information

This work was supported in part by grant C30195 from the National Institute of health, Bethesda, MD. Portion of this study appeared as a poster presentation at the Tissue Culture Association meeting, Las Vegas, 1988.

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Schneider, S.L., Fuqua, S.A.W., Speeg, K.V. et al. Isolation and characterization of an adriamycin-resistant breast tumor cell line. In Vitro Cell Dev Biol 26, 621–628 (1990). https://doi.org/10.1007/BF02624212

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  • DOI: https://doi.org/10.1007/BF02624212

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