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Effect of pH on plating efficiency, serum requirement, and incorporation of radioactive precursors into human cells

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Summary

The plating efficiencies of two cell lines (HeLa-AT and WI26 Va) and one cell strain (KL2) appeared to be optimal at pH 7.6. At the optimal pH, using organic buffers, the serum concentration could be reduced by one-half without affecting the initial growth rate or the final population density of the human skin fibroblast strain, KL2. It is suggested that an optimal pH lowers the serum requirement of the cells. The incorporation of [3H]thymidine, [3H]uridine, [3H]fucose and [14C]amino acids into KL2 was maximal at the pH which normally permits optimal growth of this cell strain. Protein turnover, as measured by loss to the medium of amino acids from prelabeled cells, was pH-independent. Thymidine incorporation did not correlated with culture growth in the cell strain, MS2A.

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  1. Department of Biological Sciences, Hunter College, C.U.N.Y., 695 Park Avenue, 10021, New York, New York

    Costante Ceccarini

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  1. Costante Ceccarini
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Supported by Grant 1580, from CUNY Research Foundation.

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Ceccarini, C. Effect of pH on plating efficiency, serum requirement, and incorporation of radioactive precursors into human cells. In Vitro 11, 78–86 (1975). https://doi.org/10.1007/BF02624079

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