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Early chromosome condensation without cell fusion

I. Preliminary results with allogeneic and xenogenic cells

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Summary

Early chromatin condensation in interphase cells (G1) of human peripheral blood lymphocytes has been induced without virus or cell fusion by exposure to allogeneic or xenogeneic mitotic cells. The event, although similar in some ways to the phenomenon described as “premature chromosome condensation”, “chromosome pulverization”, and “prophasing”, differs in that it does not require the presence of viruses and cell fusion before mitosis proceeds in the G1 cell.

Early chromatin condensation in interphase cells induced by mitotic cells only, consists of chromatids in the early or late G1 phase of the cell cycle that are not pulverized or fragmented at mitosis. Some of the chromosomes are twice as long as the metaphase chromosomes and exhibit natural bands. Almost twice as many of these bands are produced as by trypsin treatment of metaphase chromosomes. The nuclear membrane is intact and nucleoli are present, to which some chromosomes are attached. The DNA content of the precocious chromosomes in G1 is half the amount of the metaphase complement.

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References

  1. Johnson, R. T., P. N. Rao, and H. D. Hughes. 1970. Mammalian cell fusion. III. A HeLa cell inducer of premature chromosome condensation active in cells from a variety of animal species. J. Cell. Physiol. 76: 151–158.

    Article  PubMed  CAS  Google Scholar 

  2. Rao, P. N., and R. T. Johnson. 1970. Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis. Nature 225: 159–164.

    Article  PubMed  CAS  Google Scholar 

  3. Johnson, R. T., and P. N. Rao. Mammalian cell fusion: induction of premature chromosome condensation in interphase nuclei. Nature 226: 717–722.

  4. Rao, P. N., and R. T. Johnson. 1972. Premature chromosome condensation. A mechanism for the elimination of chromosomes in virus-fused cells. J. Cell Sci. 10: 495–513.

    PubMed  CAS  Google Scholar 

  5. Nichols, W. W., A. Levan, P. Aurla, and E. Norrby. 1965. Chromosome damage associated with the measles virusin vitro. Hereditas 54: 101–118.

    Article  PubMed  CAS  Google Scholar 

  6. Kato, H., and A. A. Sandberg. 1967. Chromosome pulverization in human binucleate cells following colcemid treatment. J. Cell Biol. 34: 35–45.

    Article  PubMed  CAS  Google Scholar 

  7. Matsui, S., H. Weinfeld, and A. A. Sandberg. 1971. Dependence of chromosome pulverization in virus-fused cells on events in the G2 period. J. Natl. Cancer Inst. 47: 401–409.

    PubMed  CAS  Google Scholar 

  8. Matsui, S., H. Yoshida, H. Weinfeld, and A. A. Sandberg. 1972. Induction of prophase in interphase nuclei by fusion with metaphase cells. J. Cell Biol. 54: 120–132.

    Article  PubMed  CAS  Google Scholar 

  9. Matsui, S. 1973. “Prophasing” as a possible cause of chromosome translocation in virus-fused cells. Nature New Biol. 243: 208–209.

    PubMed  CAS  Google Scholar 

  10. Matsui, S., H. Weinfeld, and A. A. Sandberg. 1972. Fate of chromatins of interphase nuclei subjected to “prophasing” in virus-fused cells. J. Natl. Cancer Inst. 49: 1621–1632.

    PubMed  CAS  Google Scholar 

  11. Stroud, A. N., R. Nathan, and K. S. Miller. 1973. Induction of premature chromosome condensation in interphase nuclei with metaphase cells (abstr.). Mammalian Chromosome Newsletter 14 (2): 81.

    Google Scholar 

  12. Stewart, C. C., and M. Ingram. 1967. A method for counting phytohemagglutinin-stimulated lymphocytes. Blood 29: 628–636.

    PubMed  Google Scholar 

  13. Puck, T. T. 1965. Phasing mitotic delay and chromosomal aberrations in mammalian cells. Science 144: 565–566.

    Article  Google Scholar 

  14. Mayall, B. H. 1969. Deoxyribonucleic acid cytophotometry of stained human leukocytes. I. Differences among cell types. J. Histochem. Cytochem. 17: 249–255.

    PubMed  CAS  Google Scholar 

  15. Castleman, K. R., and R. J. Wall. 1973. Automatic systems for chromosome identification. In: T. Caspersson, L. Zech, and V. Runnstrom-Reio (Eds.),Nobel Symposium 23-Chromosome Identification. Academic Press, London, pp. 77–84.

    Google Scholar 

  16. Caspersson, T., K. R. Castleman, G. Tomakka, E. J. Modest, A. Moller, R. Nathan, R. J. Wa, and L. Zech. 1972. Automatic karyotyping of quinacrine mustard stained human chromosomes. Exp. Cell Res. 67: 233–235.

    Article  Google Scholar 

  17. Wall, R. J. 1974. The gray level histogram for threshold boundary determination in image processing with applications to the scene segmentation problem in human chromosome analysis. (Ph.D. thesis, University of California, Los Angeles).

    Google Scholar 

  18. Hungerford, D. A., F. T. Ashton, B. B. Balaban, B. U. LaBadie, L. R. Messatizzia, G. Haller, and A. E. Miller. 1972. The C-group pachytene bivalent with a locus characteristic for parachromosomally situated particulate bodies (parameres): a provisional map in human males. Proc. Natl. Acad. Sci. U. S. A. 69: 2165–2168.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Space Sciences Division, Jet Propulsion Laboratory, 4800 Oak Grove Drive, 91103, Pasadena, California

    Agnes N. Stroud, Robert Nathan & Sayuri Harami

Authors
  1. Agnes N. Stroud
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  2. Robert Nathan
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  3. Sayuri Harami
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Additional information

This paper presents one phase of research carried out at the Jet Propulsion Laboratory, California Institute of Technology, under Research Contract NAS 7-100, sponsored by the National Aeronautics and Space Administration, and Biotechnology Resource Grant RR-00443 from the National Institutes of Health.

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Stroud, A.N., Nathan, R. & Harami, S. Early chromosome condensation without cell fusion. In Vitro 11, 61–68 (1975). https://doi.org/10.1007/BF02624077

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