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Calcium regulation of skeletal myogenesis: IV A defined culture medium permissive for myotube formation and the use of the calcium antagonist lanthanum

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Summary

Lanthanum has been used effectively in studies of calcium physiology in experiments of short duration. In experiments of longer duration, we report that solutions, such as cell culture medium, containing lanthanum (La++) undergo a decrease in pH on the time scale of hours. Presumaly, the decrease in pH is a consequece of the hydrolysis of water by the solution-active La+++ ions. We have devised a defined culture medium without serum and chick embryo extract which is permissive for myotube formation. This defined medium is also useful for studies of La+++ as a calcium antagonist. with Ca++ to low-Ca++ fusion-blocked cultures.

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Authors and Affiliations

  1. Department of Developmental Genetics and Anatomy, Case Western Reserve University, School of Medicine, 2119 Abington Road, 44106, Cleveland, Ohio

    Ronald J. Przybylski & Vilma Szigeti

  2. Department of Physiology, Case Western Reserve University, School of Medicine, 2119 Abington Road, 44106, Cleveland, Ohio

    Albert C. Kirby

Authors
  1. Ronald J. Przybylski
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  2. Vilma Szigeti
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  3. Albert C. Kirby
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Additional information

This study was supported in part by NIH grants NS 10196 and AM 25202 and The Muscular Dystrophy Association.

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Przybylski, R.J., Szigeti, V. & Kirby, A.C. Calcium regulation of skeletal myogenesis: IV A defined culture medium permissive for myotube formation and the use of the calcium antagonist lanthanum. In Vitro Cell Dev Biol 22, 402–406 (1986). https://doi.org/10.1007/BF02623530

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  • DOI: https://doi.org/10.1007/BF02623530

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