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The effect of refrigeration of bone marrow and peripheral blood on cytogenetic analysis

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Summary

Bone marrow samples from patients with various hematologic disorders were stored at 4° C for up to 5 d before the establishment of a 24-h culture. We tested various factors, including storage time, colony stimulating factor, and methotrexate in an effort to improve metaphase and chromosome quality. Cytogenetic findings for various hematologic diseases were compared in a total of 201 cultures. Cold storage for up to 3 d did not seem to adversely affect the number of mitoses or the quality of chromosome banding when cells were cultured in a system that used both colony stimulating factor and methotrexate. In samples studies in parallel, clonal abnormalities were noted as frequently in cells stored in the cold as in those processed directly.

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Author notes

  1. Paulette K. Martin

    Present address: High Tech Medical Park, 11800 S. Southwest Hwy., 60463, Palos Heights, Illinois

Authors and Affiliations

  1. Department of Medicine, University of Chicago Medical Center, 5841 S. Maryland Avenue, Box 420, 60637, Chicago, Illinois

    Paulette K. Martin & Janet D. Rowley

Authors
  1. Paulette K. Martin
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  2. Janet D. Rowley
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Additional information

This work was supported by contract DE-AC02-80EV10328 and by grant CA 16910 from the National Cancer Institute, Bethesda, MD.

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Martin, P.K., Rowley, J.D. The effect of refrigeration of bone marrow and peripheral blood on cytogenetic analysis. In Vitro Cell Dev Biol 22, 387–391 (1986). https://doi.org/10.1007/BF02623527

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  • DOI: https://doi.org/10.1007/BF02623527

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