Abstract
Cell-free extracts of 93 strains from 23 species and biotypes ofPhotobacterium, Beneckea, andVibrio were electrophoresed on polyacrylamide gels and then stained for superoxide dismutase (SOD) activity. All strains exhibited a single activity band with the exception ofPhotobacterium leiognathi, which had one major and two minor bands. Strains representative of all three genera were found to have SOD activities that were sensitive to treatment with H2O2, suggesting that they were iron-containing enzymes. Examination of the effect of gel concentration on relative mobility (Rm) suggested that all the iron-containing SODs had very similar molecular weight. Most species could be distinguished on the basis of differences in the Rm values of their SODs.Vibrio was readily separable fromBeneckea andPhotobacterium. A limited electrophoretic analysis of the SODs fromAeromonas, Serratia, Proteus, Erwinia, and other species of terrestrial enterobacteria indicated groupings that were in agreement with previous studies.
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Bang, S.S., Woolkalís, M.J. & Baumann, P. Electrophoretic mobilities of superoxide dismutases from species ofPhotobacterium, beneckea, vibrio, and selected terrestrial enterobacteria. Current Microbiology 1, 371–376 (1978). https://doi.org/10.1007/BF02621372
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DOI: https://doi.org/10.1007/BF02621372