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Culture and characterization of bovine mesenteric lymphatic endothelium

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Summary

Lymphatic endothelial cells isolated from bovine mesenteric lymphatic vessels were cultured and characterized. Lymphatic endothelial cells grew as a monolayer displaying an elongated morphology in preconfluent primary cultures. When confluent, the cells exhibited a polygonal morphology to form a “cobblestone” pattern previously described for cultured vascular endothelium. All culture lymphatic endothelial cells expressed Factor VIII-related antigen and boundUlex europaeus I lectin. Ultrastructurally, cultured lymphatic endothelium was characterized by the presence of Weibel-Palade bodies as well as the usual cytoplasmic organelles.

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Authors and Affiliations

  1. School of Pathology, University of New South Wales, Kensington, P.O. Box 1, 2033, Sydney, Australia

    B. E. Jones & L. C. J. Yong

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  1. B. E. Jones
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  2. L. C. J. Yong
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Jones, B.E., Yong, L.C.J. Culture and characterization of bovine mesenteric lymphatic endothelium. In Vitro Cell Dev Biol 23, 698–706 (1987). https://doi.org/10.1007/BF02620983

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  • DOI: https://doi.org/10.1007/BF02620983

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