Abstract
There is increasing interest in the study of the mammalian lymphatic system, including the lymphatic endothelial cells (LECs) that make up lymphatic vessels. The ability to isolate primary LECs from tissue of normal and genetically modified mice permits detailed analysis of this unique cell type. Here, we describe a robust protocol for the isolation and in vitro expansion of LECs from mouse lung by antibody-based magnetic separation.
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Lapinski, P.E., King, P.D. (2021). Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue. In: Peng, X., Zimmer, W.E. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 2319. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1480-8_8
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DOI: https://doi.org/10.1007/978-1-0716-1480-8_8
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1479-2
Online ISBN: 978-1-0716-1480-8
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