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A model for tumorigenicity and metastatic potential: Growth in 1.0% agar cultures

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We developed a method to determine the amount of work performed by cells through cell division in 1.0% agar cultures. There was no correlation between the cloning efficiencies of 1.0 and 0.3% agar cultures. Growth in 1.0% agar cultures correlated well with such malignant properties as tumorigenicity and the invasive and metastatic potentials. Our method revealed that metastatic MC and F cell lines possess different means of taking advantage of energy to proliferate against an environmental pressure from those possesed by nontumorigenic (ME and T-C3H) cell strain/line or nonmetastatic but tumorigenic (L, MR, and magc1) cell lines.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine, Kyoto University, 606, Kyoto, Japan

    T. Saiga, T. Ohbayashi, K. Tabuchi & O. Midorikawa

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  1. T. Saiga
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  2. T. Ohbayashi
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  3. K. Tabuchi
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  4. O. Midorikawa
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Saiga, T., Ohbayashi, T., Tabuchi, K. et al. A model for tumorigenicity and metastatic potential: Growth in 1.0% agar cultures. In Vitro Cell Dev Biol 23, 850–854 (1987). https://doi.org/10.1007/BF02620964

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  • DOI: https://doi.org/10.1007/BF02620964

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