Summary
When primary cultures of fetal human liver cells established on type I collagen gels were compared to sister cultures developed on tissue culture plastic, the cells in contact with type I collagen secreted albumin at a higher rate than those without contact. The albumin secretion was dependent on the presence of hydrocortisone (HC) in the medium. Also, α-fetoprotein (AFP), of which the level decreased gradually and became undetectable after 6 d regardless of the presence or absence of HC in the cells cultured on plastic, was maintained for longer periods of time by plating the cells on type I collagen gels in the presence of HC. Different secretion rates of albumin and AFP were observed after Day 13 and Day 16, respectively, between cells maintained on type I collagen gels and those on film plastic. The cells secreted larger amounts of both albumin and AFP in plates coated with type IV or I collagens than with fibronectin after Day 10. The cells cultured on type I collagen gels were cuboidal in shape, whereas those on plastic were flattened in cultures with HC. These data indicate that the secretion of human albumin and AFP is facilitated by synergies between HC and collagenous substrata.
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Baffet, G.; Clement, B.; Glaise, D., et al. Hydrocortisone modulates the production of extracellular material and albumin in long-term cocultures of adult rat hepatocytes with other liver epithelial cells. Biochem. Biophys. Res. Commun. 109:507–512; 1982.
Belanger, L.; Hamel, D.; Lachance, L., et al. Hormonal regulation of α1-fetoprotein. Nature 256:657–659; 1975.
Bissell, D. M.; Tilles, J. G. Morphology and function of cells of human embryonic liver in monolayer culture. J. Cell. Biol. 50:222–231; 1971.
Bissell, M. J.; Hall, G. H.; Parry, G. How does the extracellular matrix direct gene expression? J. Theoret. Biol. 99:31–68; 1982.
Brown, P. C.; Papaconstantinou, J. Coordinated modulation of albumin synthesis and mRNA levels in cultured hepatoma cells by hydrocortisone and cyclic AMP analogs. J. Biol. Chem. 254:9379–9384; 1979.
Chou, J. Y. Temperature-sensitive adult liver cell line dependent on glucocorticoid for differentiation. Mol. Cell. Biol. 3:1013–1020; 1983.
Comer, P.; Schwaltz, C.; Tracy, S., et al. Dexamethasone inhibits α-fetoprotein gene expression in developing mouse liver. Biochem. Biophys. Res. Commun. 89:1294–1299; 1979.
Emerman, J. T.; Enami, J.; Pitelka, D. R., et al. Hormonal effects on intracellular and secreted casein in cultures of mouse mammary epithelial cells on floating collagen membranes. Proc. Natl. Acad. Sci. USA 74:4466–4470; 1977.
Freeman, A. E.; Engvall, E.; Hirata, K., et al. Differentiation of fetal liver cells in vitro. Proc. Natl. Acad. Sci. USA 78:3659–3663; 1981.
Gatmaitan, Z.; Jefferson, D. M.; Ruiz-Opazp, N., et al. Regulation of growth and differentiation of a rat hepatoma cell line by the synergistic interactions of hormones and collagenous substrata. J. Cell. Biol. 97:1179–1190; 1983.
Kleinman, H. K.; Klebe, R. J.; Martin, G. R. Role of collagenous matrices in the adhesion and growth of cells. J. Cell Biol. 88:473–485; 1981.
Laishes, B. A.; Williams, G. M. Conditions affecting primary cell cultures of functional adult rat hepatocytes. II. Dexamethasone-enhanced longevity and maintainence of morphology. In Vitro 12:821–832; 1976.
Michalopoulos, G.; Pitot, H. C. Primary culture of parenchymal liver cells on collagen membranes. Exp. Cell Res. 94:70–78; 1975.
Murray, J. C.; Stingl, G.; Kleinman, H. K., et al. Epidermal cells adhere preferentially to type IV (basement membrane) collagen. J. Cell Biol. 80:197–202; 1979.
Nechaud, B.; Becker, J. E.; Potter, V. R. Effect of glucocorticoids on fetoprotein production by an established cell line from Morris hepatoma 8994. Biochem. Biophys. Res. Commun. 68:8–15; 1976.
Ruoslahti, E.; Seppala, M. α-Fetoprotein in cancer and fetal development. Adv. Cancer Res. 29:275–346; 1979.
Tokiwa, T.; Miyagiwa, M.; Sato, J. An attempt to eliminate fibroblast-like cells from primary cultures of fetal human livers. Acta Med. Okayama 40:107–111; 1986.
Tokiwa, T.; Nakabayashi, H.; Miyazaki, M., et al. Isolation and characterization of diploid clones from adult and newborn rat liver cell lines. In Vitro 15:393–400; 1979.
Uotila, M.; Ruoslahti, E.; Engvall, E. Two site sandwich enzyme immunoassay with monoclonal antibodies to human alphafetoprotein. J. Immunol. Methods 42:11–13; 1981.
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Tokiwa, T., Taketa, K. & Sato, J. Production of albumin and α-fetoprotein in primary culture of fetal human liver cells on collagenous substrata in the presence of hydrocortisone. In Vitro Cell Dev Biol 23, 830–836 (1987). https://doi.org/10.1007/BF02620961
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DOI: https://doi.org/10.1007/BF02620961