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Enhancement of rat ACT-1 tumor clonogenicity by xenogeneic mouse macrophages

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Summary

In vitro growth of rat atriocaval epithelial tumor cells (ACT-1) was enhanced by the inclusion of xenogeneic mouse adherent peritoneal exudate cells (PECs) in a two-layer soft agar system. A linear relationship was found between the number of cells plated and the number of colonies when ACT-1 tumor cells were plated at plating densities of between 1 and 5×105 cell/60 mm plate (r=0.9,P<0.001). Inclusion of irradiated PECs in the bioassay for tumor stem cells resulted in a two and a half-fold increase in colony formation in three separate experiments (P<0.001).

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Authors and Affiliations

  1. Cancer Research Unit, University of Otaga Medical School, P.O. Box 913, Dunedin, New Zealand

    K. W. Beagley, L. S. Horne, R. F. X. Noronha, C. M. Goodall & C. M. Moore

Authors
  1. K. W. Beagley
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  2. L. S. Horne
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  3. R. F. X. Noronha
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  4. C. M. Goodall
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  5. C. M. Moore
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Additional information

This work was supported by grants from the Cancer Research Trust, the University of Otago Cancer Research Fund and by the Medical Research Committee (Golden Kiwi).

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Beagley, K.W., Horne, L.S., Noronha, R.F.X. et al. Enhancement of rat ACT-1 tumor clonogenicity by xenogeneic mouse macrophages. In Vitro 20, 623–628 (1984). https://doi.org/10.1007/BF02619611

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  • DOI: https://doi.org/10.1007/BF02619611

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