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Biological characteristics and viral susceptibility of a stable dog kidney cell line

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Summary

A cell line derived in 1956 from normal dog kidney is described. The cells are epithelial, contact-inhibited, and can be maintained in the same culture vessels for periods of more than 2.5 yr. Karyologically, the cells are hypodiploid with a modal number of 72 as opposed to the diploid number of 78. The karyotype indicates male origin of the cells and clonal derivation of extant cultures due to the presence of two marker chormosomes in all metaphases observed. At the 159th passage the dog kidney (DK) cells did not produce tumors in athymic rats. At least 13 viruses of various types produced transmissible cytopathogenic effects in the DK cells, including all of the human influenza viruses investigated.

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Authors and Affiliations

  1. Life Sciences Division, SRI International, 94025, Menlo Park, California

    A. Howard Fieldsteel & M. Joseph Colston

  2. Cell Culture Laboratory, School of Public Health, Naval Biosciences Laboratory, University of California, 94624, Oakland, California

    Walter A. Nelson-Rees

Authors
  1. A. Howard Fieldsteel
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  2. Walter A. Nelson-Rees
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  3. M. Joseph Colston
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Additional information

The project was supported in part by an Institutional Research and Development grant from SRI International and Contract Y01 CP8-0500, National Cancer Institute, National Institutes of Health, Bethesda, MD.

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Fieldsteel, A.H., Nelson-Rees, W.A. & Colston, M.J. Biological characteristics and viral susceptibility of a stable dog kidney cell line. In Vitro 18, 220–226 (1982). https://doi.org/10.1007/BF02618574

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  • DOI: https://doi.org/10.1007/BF02618574

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