Abstract
A wide range of influenza B virus strains with various passage histories uniformly formed well-defined clear plaques with high efficiency in cultures of an established line of canine kidney cells (MDCK). PFU titers of the viruses assayed in MDCK exceeded the titers assayedin ovo. With recently isolated strains such as B/Hong Kong/5/72 and Gifu/2/73, the PFU/EID50 ratios were as high as 100 to 400. MDCK cells have been successfully employed for primary isolation of influenza B viruses from throat washings of patients by direct plaquing.
Similar content being viewed by others
References
Babiker, H. A., Rott, R.: Plaque formation by influenza viruses in monolayers of chicken kidney cells. J. gen. Virol.3, 285–287 (1968)
Beare, A. S., Keast, K. A.: Influenza virus plaque formation in different species of cell monolayers. J. gen. Virol.22, 347–354 (1974)
Gaush, C. R., Smith, T. F.: Replication and plaque assay of influenza virus in an established line of canine kidney cells. Appl. Microbiol.16, 588–594 (1964)
Lehmann-Grube, F.: A sensitive plaque assay for influenza viruses. Virology21, 520–522 (1963)
Mizutani, H., Nagase, M.: Plaque formation with influenza viruses in explants of chick embryo fibroblasts from embryos of different ages. Arch. ges. Virusforsch.20, 278–282 (1967)
Tobita, K., Kilbourne, E. D.: Genetic recombination for antigenic markers of antigenically different strains of influenza B virus. J. Virol.13, 347–352 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tobita, K. Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B viruses. Med Microbiol Immunol 162, 23–27 (1975). https://doi.org/10.1007/BF02123574
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02123574