Summary
When rat marrow cells are pre-incubated for periods of up to 20 days they retain the capacity to respond to exogenous erythropoietin. This is true whether the response is measured in terms of increased rates of RNA synthesis, protein synthesis, iron-uptake, or hemoglobin synthesis. The patterns of response to erythropoietin by pre-incubated cells are complex and involve secondary waves of increased rates of synthesis that are not yet understood.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Goldwasser, E. 1966. Biochemical control of erythroid cell development, p. 173–211.In A. Monroy and A. Moscona Eds.,Current Topics in Developmental Biology, Volume 1, Academic Press, New York.
Goldwasser, E. and Kung, C. K. H. 1968. Progress in the purification of erythropoietin. Ann. N. Y. Acad. Sci.,149: 49–53.
Mel, H. C. and Schooley, J. C. 1964. Stable-flow free boundary fractionation of spleen colony-forming cells from mouse bone marrow. Exp. Hematol.,7: 26–29.
Turner, R. W. A., Siminovitch, L., Mcculloch, E. A. and Till, J. E. 1967. Density gradient centrifugation of hemopoietic colony-forming cells. J. Cell Physiol.,69: 73–82.
Takaku, F., Dukes, P. P. and Goldwasser, E. 1964.In vitro studies of cell types responsive to erythropoietin. Endocrinology74: 968–972.
Krantz, S. B. and Goldwasser, E. 1965. On the mechanism of erythropoietin-induced differentiation. II. The effect on RNA synthesis. Biochim. Biophys. Acta,103: 325–332.
Gross, M. and Goldwasser, E. 1968. Effect of erythropoietin on RNA synthesis. Fed. Proc.,27: 394.
Krantz, S. B., Gallien-Lartigue, O. and Goldwasser, E. 1963. The effect of erythropoietin upon heme synthesis by marrow cellsin vitro. J. Biol. Chem.,238: 4085–4090.
Gallien-Lartigue, O. and Goldwasser, E. 1964. Hemoglobin synthesis in marrow cell culture: the effect of rat plasma on rat cells. Science145: 277–279.
Dukes, P. P., Takaku, F. and Goldwasser, E. 1964.In vitro studies on the effect of erythropoietin on glucosamine-1-14C incorporation into rat bone marrow cells. Endocrinology74: 960–967.
Hrinda, M. E. and Goldwasser, E. 1966. Facilitated iron entry into marrow cells: an induced permease-like activity. Fed. Proc.,25: 284.
Winzler, R. J., Harris, E. D., Pekas, D. J., Johnson, C. A. and Weber, P. 1967. Studies on glycopeptides released by trypsin from intact human erythrocytes. Biochemistry6: 2195–2207.
Borsook, H. 1964. DNA, RNA and protein synthesis after acute, severe blood loss. Ann. N. Y. Acad. Sci.,119: 523–539.
Hrinda, M. and Goldwasser, E., unpublished data.
Goldwasser, E., White, W. F. and Taylor, K. B. 1962. Further purification of sheep plasma erythropoietin. Biochim. Biophys. Acta,64: 487–496.
Author information
Authors and Affiliations
Additional information
Life Insurance Medical Research Fund Fellow.
Operated by the University of Chicago for the United States Atomic Energy Commission.
Rights and permissions
About this article
Cite this article
Goldwasser, E., Gross, M. Some studies of erythroid differentiationin vitro . In Vitro 4, 36–44 (1969). https://doi.org/10.1007/BF02618209
Issue Date:
DOI: https://doi.org/10.1007/BF02618209