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Continuous observation diffusion chamber for cell and organ cultures

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Summary

A diffusion chamber which allows the continuous observation of biological indicators is described. Cells can be microscopically monitored while they are exposed to soluble products diffusing across a semipermeable membrane. The chamber consists of a cylinder of selective membrane material, in which the indicator system is contained, bonded to a normal tissue culture dish. Various cell cultures (HeLa, HT29, L cells) and organ cultures (hamster trachea) have been propagated successfully in these dishes. This continuous observation diffusion chamber is simple to construct and manipulate, and appears to have widespread potential application.

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Authors and Affiliations

  1. The Department of Microbiology, School of Basic Medical Sciences, The University of Illinois, 61801, Urbana, Illinois

    Michael G. Gabridge

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  1. Michael G. Gabridge
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Additional information

Supported in part by contract DADA 17-73-C-3016 from the United States Army Medical Research and Development Command.

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Gabridge, M.G. Continuous observation diffusion chamber for cell and organ cultures. In Vitro 9, 455–457 (1974). https://doi.org/10.1007/BF02615998

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  • DOI: https://doi.org/10.1007/BF02615998

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