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Measuring Pericellular Oxygen Tension for In Vitro Cell Culture

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Hypoxia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2755))

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Abstract

Cell culture, the process of growing cells in conditions that mimic those in the body, is a key technique in biomedical research. Oxygen is not controlled in conventional cell culture, although chambers that control oxygen in the surrounding gas phase are commercially available. In both cases, it is valuable to understand the pericellular oxygen tension (i.e., the oxygen concentration that cells experience) in cultures. Herein we describe a procedure for using commercial optical sensor spots to measure pericellular oxygen for adherent and suspension cultures. Spots are placed on surfaces on which cells are grown, and optical cables are attached to the outside of the cell culture vessels and connected to a computer. Associated software allows for the real-time monitoring of pericellular oxygen during cell culture experiments. This procedure enhances the reproducibility and control of cell culture.

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Acknowledgments

This work was financially supported by the National Institutes of Health (1R01EB027705) and the National Science Foundation (DMR 1847843, TI 2141019).

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Authors and Affiliations

  1. Department of Chemical Engineering, Northeastern University, Boston, MA, USA

    Zachary J. Rogers & Sidi A. Bencherif

  2. Department of Pharmaceutical Sciences, Northeastern University, Boston, MA, USA

    Khushbu Bhatt

  3. Department of Bioengineering, Northeastern University, Boston, MA, USA

    Sidi A. Bencherif

  4. Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA

    Sidi A. Bencherif

  5. Biomechanics and Bioengineering (BMBI), UTC CNRS UMR 7338, University of Technology of Compiègne, Sorbonne University, Compiègne, France

    Sidi A. Bencherif

Authors
  1. Zachary J. Rogers
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  2. Khushbu Bhatt
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  3. Sidi A. Bencherif
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Corresponding author

Correspondence to Sidi A. Bencherif .

Editor information

Editors and Affiliations

  1. Department of Oncology Johns Hopkins, University School of Medicine, Baltimore, MD, USA

    Daniele M. Gilkes

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Rogers, Z.J., Bhatt, K., Bencherif, S.A. (2024). Measuring Pericellular Oxygen Tension for In Vitro Cell Culture. In: Gilkes, D.M. (eds) Hypoxia. Methods in Molecular Biology, vol 2755. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3633-6_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3633-6_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3632-9

  • Online ISBN: 978-1-0716-3633-6

  • eBook Packages: Springer Protocols

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