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Prolonged cultivation of primary chick cultures using organic buffers

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Summary

Primary cultures of chick embryos have been carried beyond the 50th passage in cell culture media containingN-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES). The doubling, potential, was found to decrease twice as fast for chick cells as it did for WI-38 human cells.

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Authors and Affiliations

  1. Masonic Medical Research Laboratory, Bleecker Street, 13501, Utica, New York

    H. R. Massie, M. B. Baird & H. V. Samis

Authors
  1. H. R. Massie
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  2. M. B. Baird
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  3. H. V. Samis
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Massie, H.R., Baird, M.B. & Samis, H.V. Prolonged cultivation of primary chick cultures using organic buffers. In Vitro 9, 441–444 (1974). https://doi.org/10.1007/BF02615996

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  • DOI: https://doi.org/10.1007/BF02615996

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