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Effects of growth factors during in vitro culture of mouse and rat embryos

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Russian Journal of Genetics: Applied Research

Abstract

The development of embryos of the ICR, HT1AN/lcgn, HT1AC/lcgn, and C57BL/6J-Ay mouse strains, as well as the OXYS/lcgn rat strains, in the culture in vitro was studied under the influence of the granulocyte- macrophage colony stimulating factor (GM-CSF) and epidermal growth factor (EGF). Both mice and rats embryos were initially frozen according to the standard protocol of the freezing program using glycerol and sucrose as cryoprotectant and culture in the rat 1-cell embryo culture medium (R1ECM) for either 24 h (mice) or 72 h (rats) after thawing. The effects of the growth factors were studied on 8-cell embryos in mice and 2–4-cell embryos in rats. The GM-CSF effect resulted in an increase in the percentage of developing embryos in the mice of both strains (HT1AC/lcgn and C57BL/6J-Ay); at the same time, no EGF effect on the mice embryos was found. The situation was the opposite for rats. The EGF effect resulted in the acceleration of the development until the stage of blastocyst in the OXYS/lcgn strain rats, but no GM-CSF effect on the rat embryos was found. The acceleration of the development is observed during the joint culture of 4-cell embryos of the HT1AN/lcgn mice strain with later stages of development (embryos at the morula stage) of the ICR strain. The results of the presented experiments indicate a species specificity of the effect of the growth factors on the mice and rats embryos, as well as demonstrating that joint culture of embryos of the later stages (morula) of mice with earlier stages (4-cell embryos) has a stimulating effect on the latter.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    E. Yu. Brusentsev, T. N. Igonina, I. N. Rozhkova, D. S. Ragaeva & S. Ya. Amstislavsky

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  1. E. Yu. Brusentsev
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  2. T. N. Igonina
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  3. I. N. Rozhkova
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  4. D. S. Ragaeva
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  5. S. Ya. Amstislavsky
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Correspondence to E. Yu. Brusentsev.

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Original Russian Text © E.Yu. Brusentsev, T.N. Igonina, I.N. Rozhkova, D.S. Ragaeva, S.Ya. Amstislavsky, 2015, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2015, Vol. 19, No. 4, pp. 372–377.

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Brusentsev, E.Y., Igonina, T.N., Rozhkova, I.N. et al. Effects of growth factors during in vitro culture of mouse and rat embryos. Russ J Genet Appl Res 6, 378–383 (2016). https://doi.org/10.1134/S2079059716040055

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  • DOI: https://doi.org/10.1134/S2079059716040055

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