Skip to main content
SpringerLink
Log in

The effects of the buffer hepes on the division potential of WI-38 cells

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

N-2-Hydroxyethylpiperazine-N′-2-ethane sulfonic acid (HEPES) and N-tris(hydroxymethyl)methyl-2-aminoethane sulfonic acid (TES) have been found to be nontoxic substitutes for bicarbonate buffer in cell culture media. WI-38 embryonic human lung cells have been carried beyond the 60th passage with both HEPES and TES buffers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hayflick, L., and P. S. Moorhead. 1961. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585–621.

    Article  Google Scholar 

  2. Carrel, A., and A. H. Ebeling. 1921. Age and multiplication of fibroblasts. J. Exp. Med. 34: 599–623.

    Article  Google Scholar 

  3. Hay, R. J., and B. L. Strehler. 1967. The limited growth span of cell strains isolated from the chick embryo. Exp. Gerontol. 2: 123–135.

    Article  Google Scholar 

  4. Simons, J. W. I. M. 1970. A theoretical and experimental approach to the relationship between cell variability and agingin vitro. In: E. Holeckova and V. Cristofalo (Eds.),Aging in Cell and Tissue Culture. Plenum Press, New York, pp. 25–39.

    Google Scholar 

  5. Martin, G. M., C. A. Sprague, and C. J. Epstein. 1970. Replicative life-span of cultivated human cells. Lab. Invest. 23: 86–92.

    PubMed  CAS  Google Scholar 

  6. Puck, T. T., S. J. Cieciura, and A. Robinson. 1958. Genetics of somatic mammalian cells. III. Long term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945–955.

    Article  PubMed  CAS  Google Scholar 

  7. Shipman, C.. 1969. Evaluation of 4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid (HEPES) as a tissue culture buffer. Proc. Soc. Exp. Biol. Med. 130: 305–310.

    PubMed  CAS  Google Scholar 

  8. Good, N. E., G. D. Winget, W. Winter, T. N. Connolly, K. Izaua, and R. M. M. Singh. 1966. Hydrogen ion buffers for biological research. Biochemistry 5: 467–477.

    Article  PubMed  CAS  Google Scholar 

  9. Hayflick, L. 1971. Personal communication.

Download references

Author information

Authors and Affiliations

  1. Masonic Medical Research Laboratory, Bleecker Street, 13501, Utica, New York

    H. R. Massie, H. V. Samis & M. B. Baird

Authors
  1. H. R. Massie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. V. Samis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. B. Baird
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Massie, H.R., Samis, H.V. & Baird, M.B. The effects of the buffer hepes on the division potential of WI-38 cells. In Vitro 7, 191–194 (1972). https://doi.org/10.1007/BF02615974

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02615974

Keywords

Search

Navigation