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Cultivation in vitro of organs of the fresh water gastropod prosobranch molluscviviparus viviparus L

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Summary

Many organs ofViviparus viviparus were explanted into a culture medium of low osmotic pressure, adapted for fresh water molluscs. The development in vitro of the explants differed according to the particular organ. The best results were obtained with the heart, which was still contracting after 2 months in culture, and with the tentacle, which remained excitable for a period of 6 to 7 weeks. The central nervous system survived well for 18 to 20 days. The fragments of digestive gland died rapidly, in about 1 week.

Fragments of intestine retained a normal appearance for 2 weeks. The period of survival of male or female gonads was also 2 to 3 weeks. Vitellogenesis did not proceed very far in the ovary, but the young oocytes remained healthy. In the testis, only spermatogonia persisted and continued to divide during the culture period: further stages would appear to require a hormonal supplement.

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  1. Laboratoire de Zoologie et Embryologie, Equipe de Recherche associée au C.N.R.S. No. 229, Faculté des Sciences, Place Maréchal Leclerc, 25030, Besançon Cedex, France

    B. Griffond & L. Gomot

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  1. B. Griffond
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  2. L. Gomot
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Griffond, B., Gomot, L. Cultivation in vitro of organs of the fresh water gastropod prosobranch molluscviviparus viviparus L. In Vitro 10, 206–215 (1974). https://doi.org/10.1007/BF02615234

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