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Studies on deoxyribonucleic acid polymerase from normal and down’s syndrome skin fibroblasts in vitro

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Summary

This investigation showed that: (a) hypotonic pH 5.0 extracts of normal and Down’s syndrome fibroblasts cells (DS) exhibit DNase activity which appears to be very similar in nature and extent; (b) DNA polymerase activity was generally increased in DS cells when compared with normal cells; (c) DNA polymerase activity was increased with increased passage age for both normal and DS fibroblasts, but the normal fibroblast was never found to overlap with DS cell activity; and (d) normal cells appear to have a similar DNA polymerase activity at all chronological ages, whereas DS cells exhibit a decrease in activity with increased age of the donor.

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Authors and Affiliations

  1. Department of Pediatrics, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada

    Russell E. Giblak & Ernest E. McCoy

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  1. Russell E. Giblak
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  2. Ernest E. McCoy
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Giblak, R.E., McCoy, E.E. Studies on deoxyribonucleic acid polymerase from normal and down’s syndrome skin fibroblasts in vitro. In Vitro 10, 167–171 (1974). https://doi.org/10.1007/BF02615229

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