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Isolation and rapid screening of deletion mutants of temperateBacillus subtilis molecular cloning vehicle ρ14

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Abstract

Four deletion mutants ofBacillus subtilis temperate bacteriophage ρ14 have been isolated by selection with the chelating agent tetrasodium pyrophosphate. The presence of deletions in the genome are readily detected by restriction endonuclease digestion patterns on agarose gels. The deletion mutants have been characterized as to their resistance to the killing effect of tetrasodium pyrophosphate and their buoyant densitiese have been determined in cesium chloride density gradients. Three of the deletion mutants, designated Φdoc26, Φdoc36 and Φdoc39, are defective in the establishment of stable lysogens and form clear plaques on a sensitive host. The fourth delection mutant, designated Φdo7, still retains the ability to establish lysogeny.

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  1. Department of Microbiology, The Ohio State University, 43210, Columbus, Ohio, USA

    James M. Kroyer & Donald H. Dean

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  1. James M. Kroyer
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  2. Donald H. Dean
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Kroyer, J.M., Dean, D.H. Isolation and rapid screening of deletion mutants of temperateBacillus subtilis molecular cloning vehicle ρ14. Current Microbiology 3, 45–50 (1979). https://doi.org/10.1007/BF02603133

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